ch 7 Flashcards

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1
Q

autoclave

A

steam heat under pressure- steam must contact item’s surface

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2
Q

pasteurization

A

reduce, not sterilize spoilage organisms
high temp for a short time
-ultra past.- very high temp for even shorter time

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3
Q

dry heat/ flamming

A

kills by oxidation

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4
Q

biocide/germicide

A

kills microbes

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5
Q

bacteriostasis

A

inhibiting, not killing microbes

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6
Q

asepsis

A

the absence of significant contamination

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7
Q

sterilization

A

removing all microbial life

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8
Q

disinfection

A

removing pathogens

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9
Q

degerming

A

removing microbes from a limited area

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10
Q

sanitization

A

lowering microbial counts on eating utensils

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11
Q

filtration

A

decreases number of microbes based on size of microbes, used when you can’t use heat

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12
Q

low temp

A

inhibits microbial growth- refridgeration, deep freeze, lyophilization

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13
Q

high pressure

A

denatures proteins

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14
Q

desiccation

A

prevents metabolism

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15
Q

osmotic pressure

A

causes plamolysis

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16
Q

microbial death curve

A

kill 90%

17
Q

how does radiation kill cells

A

damages the DNA- ionizing and nonionizing

18
Q

List the 4 factors related to effective disinfection

A
  1. concentration of disinfectant
  2. organic matter
  3. pH
  4. time
19
Q

interpret the results of the disk-diffusion method

A

will have a zone of inhibition- does not kill microbes, just slows the growth

20
Q

list the most to least resistant microbes (1-5)

A
  1. prions
  2. endospores of bacteria
  3. mycobacteria
  4. cysts of protozoa
  5. vegitative protozoa
21
Q

list the most to least resistant microbes (6-10)

A
  1. gram-neg bacteria
  2. fungi (including spores)
  3. vius w envelopes
  4. gram pos bacteria
  5. virus w lipid envelopes
22
Q

which is best way to get rid of microbes

A

sterilization- moist heat

23
Q

thermal death point

A

lowest temp at which all cells in a culture are killed in 10 min

24
Q

thermal death time

A

time during which all cells in a culture are killed

25
Q

decimal reduction time

A

min to kill 90% of a population at a given temp

26
Q

triclosan

A

a bisphenol (disrupts plasma membranes)