Bones and Joints Flashcards
Primitive osteoprogenitor cells of mesenchymal origin develop into all of the following cell types in bones except: A. osteoblasts B. osteocytes C. lining cells D. osteoclasts
D. osteoclasts. Osteoclasts are derived from hematopoietic stem cells of the macrophage line
Which transcription factor guides development of mesenchymal cells into osteoprogenitor cells?
Runx2 ( runt-related transcription factor 2)
SOC5, 6 and 9 are responsible for development of which cell type?
Chondrocytes
PPAR-gamma2 (peroxisome proliferator activator receptor) is responsible for development of which cell type?
Adipocytes
Osteoblasts develop under signaling from which transcription factors?
Runx2, osterix, beta-catenin
Functional roles of osteoblasts include?
manufacturing osteoid, initiate mineralization, mediate both bone resorption and formation, produce regulatory factors deposited in the bone matrix
Bone lining cells are inactive versions of what cells?
osteoblasts
Which type of bone cell is responsible for lining the endosteal surface of bone and forming the functional barrier between the extracellular fluid compartment of bone tissue and surrounding tissues?
bone lining cells, most abundant cell type in the endosteal skeleton. Form barrier between osteoclasts precursors and bone surface.
What cells are responsible for the bone-blood barrier, controlling the movement of ions in and out of the extracellular fluid?
bone-lining cells
What is an osteoblast marker expressed by bone-lining cells during active remodeling?
RANKL ( receptor activator of nuclear factor kappa B ligand)
When does an osteoblast become and osteocyte?
When it surrounds itself with osteoid and resides in lacunae in the mineralized matrix
How do osteocytes maintain contact with other osteocytes, osteoblasts and bone-lining cells?
Through a network of branching cytoplasmic processes called canaliculi
What key regulatory factors for bone resorption and formation are produced by osteocytes?
sclerostin, RANKL, fibroblast growth factor 23 (FGF23), All produced under control of PTH
What is the function of increased RANKL expression by osteocytes?
Osteoclastic bone resorption
What is the function of FGF23 expression by osteocytes?
phosphate homeostasis –> phosphate excretion from the kidney
What are the key factors involved in osteoclast formation?
macrophage colony stimulating factors and RANK (not RANKL - that’s produced by osteoblasts and osteocytes). RANKL from osteoblasts/cytes allows fusion of osteoclast precursors to form the mature osteoclast.
IHC for which antigen can be useful to identify osteoclasts?
TRAP (tartrate resistant acid phosphatase)
Howship’s lacunae are shallow pits on bone surfaces containing what?
mature osteoclasts. Their presence indicates previous resorption even if the osteoclast is gone.
What factors can enhance osteoclast survival?
Inflammatory cytokines like TNF-a and IL-1 as well as PTH. Explains why osteoclasts are found within medullary spaces filled with fibrous tissue
What type of collagen is found in bone?
Type I collagen. Also the main collagen found in tendons, ligaments, ocular sclera and dentin
The enzyme lysyl oxidase which is responsible for the intermolecular cross-links in collagen, form with the assistance of what mineral?
Copper
What noncollagenous protein is produced by osteoblast, found in bone and is responsible for interacting with both type I collagen and hydroxyapatite?
Osteonectin or SPARC. May be important in intramembranous bone formation post-fracture
The production of osteocalcin (or bone-Gla protein) by osteoblasts is dependent on what vitamin? What vitamin causes an increase in its synthesis?
dependent on Vitamin K, Vitamin D increases synthesis
What is the function of osteocalcin in bone formation?
when carboxylated, osteocalcin is deposited in osteoid before mineralization and allows strong binding to calcium