BIOL 435 Ch. 3 Part Three (Recognition and Response) Flashcards

1
Q

PRRs

A
  • pattern recognition receptors
  • immune An receptor molecules
  • recognize PAMPs
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2
Q

PAMPs

A
  • pathogen associated molecular patterns

- represent motifs of recurring patterns on bacteria, yeast and parasites

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3
Q

receptors for PAMPs

A
  • may uniformly recognize large numbers of bacteria that share the same PAMPs
  • not clonally distributed, but are expressed equally on the same cell types
  • may be integral membrane proteins or intracellular proteins
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4
Q

PRR families

A
  1. Toll-like receptor
  2. C-type lectin receptor
  3. Retinoic acid inducible gene-1 like receptor
  4. Nucleotides oligoermizatlon domain like receptor
  5. Absent-in-melanoma-like receptor
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5
Q

toll-like receptor (TLR)

A
  • plasma membrane, endosomes, or lysosomes
  • production of antimicrobials, antivirals and cytokines
  • inflammation
  • various ligands (microbial CHO, bacterial flagellin, viral RNA)
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6
Q

C-type lectin receptor (CLR)

A
  • plasma membrane
  • bind to CHO components of various things
  • phagocytosis
  • production of antimicrobials and cytokines
  • inflammation
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7
Q

cytokine signal

A
  • usually generate by the binding of a ligand to a complementary cell-bound receptor
  • any event where a chemical messenger binds to a receptor with the result that the cell is instructed to change its metabolic or proliferative state
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8
Q

cytokine-receptor binding

A

-non-covalent

>may be of generally high affinity

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9
Q

cytokine-signalling end results

A

-often induce a change in the transcriptional program of the target cell

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10
Q

cytokines

A

-proteins that mediate the effector functions of the immune system
-can act in several ways:
>endocrine action
>paracrine action
>autocrine action

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11
Q

endocrine action

A
  • released into the blood stream to effect distant cells

- sometimes

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12
Q

paracrine action

A
  • released to effect nearby cells

- very common

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13
Q

autocrine action

A
  • released, but then bind to receptors on the cell that produced them
  • less common
  • very important way for immune cells to be activated or be more activated
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14
Q

general properties of cytokines and chemokines

A
  • pleiotrophic activity
  • redundant activity
  • synergy effect
  • antagonist effect
  • cascade effect
  • combination of molecules will determine what will occur
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15
Q

pleiotrophic activity

A

-induces different biological effect dependent on target cell

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16
Q

redundant activity

A

-mediates similar effects on target cell

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17
Q

synergy effect

A

-combines 2 cytokine activities to be greater than additive effect

18
Q

antagonist effect

A

-inhibits one cytokines effect by another’s action

19
Q

cascade effect

A

-of one cytokine or one target cell to produce additional cytokines

20
Q

6 major cytokine families

A
  1. Interleukin-1 family
  2. Class 1 (hematopoietin)
  3. Class 2 (interferon)
  4. Tumor necrosis factor family
  5. Interleukin-17 family
  6. Chemokines
21
Q

interleukin-1 family

A
  • first non-interferon cytokine

- important inflammatory mediators

22
Q

class 1 family

A
  • large family of small cytokine molecules

- sequence and functional diversity

23
Q

class 2 family

A
  • antiviral responses
  • important modulators of immune responses
  • send ‘save yourself’ signals
24
Q

tumor necrosis factor family

A
  • soluble or membrane bound

- immune system development, effector functions and homeostasis

25
Q

interleukin-17 family

A
  • most recently discovered
  • promote neutrophil accumulation and activation
  • pro-inflammatory
26
Q

chemokines

A

-all serve chemoattractant function
>attract other immune cells
-direct leukocyte migration

27
Q

cytokine receptors

A
  • can have common subunits used by different cells in different ways
  • pairing of subunits for different molecules and responses
28
Q

chemokine structure

A
  • small proteins

- process highlty conserved disulfide bonds that dictate both structure and category

29
Q

leukocyte migration

A

-signalling through chemokine receptors help cells move to different body areas

30
Q

chemokine receptors

A
  • examples of GPCR
  • many can bind to more than 1 chemokine
  • several chemokines are able to bind to more than one receptor
31
Q

cellular signal

A

-any event that instructs a cell to change its metabolic or proliferative state

32
Q

singals are usually generated by

A

-the binding of a ligand to a complementary cell-bound receptor

33
Q

expression of the receptor

A

-cell can become more or less susceptible to actions of a ligand by increasing or decreasing expression

34
Q

induces a change

A

-cell signalling often does that in the transcriptional program of the target cell

35
Q

multiple signals through multiple receptors

A

-are sometimes required to effect particular outcomes

36
Q

integration of all signals

A

-received by a cell occurs at the molecular level inside the recipient cell

37
Q

receptors and cell signalling

A

-ligand binding induces a variety of downstream effects, many of which culminate in transcription factor activation

38
Q

An- mediated receptor clustering

A

-initiates signalling in B and T cells
-receptor dimerization is often a result
>multimerization can also occur
-clustered receptors are localized in lipid rafts

39
Q

lipid rafts

A
  1. Lots of cholesterol
  2. Straight FA tails=packed
    >wont be as mobile and can pass the signal longer
40
Q

tyrosine phosphorylation

A
  • an early step in many signalling pathways
  • CD3 (T-cells) and Ig alpha/beta (B-cells) are phosphorylated on ITAMs
  • phosphorylated tyrosines serve as docking points for adapter molecules
41
Q

Class 1+2 cytokines receptors

A
  1. Cytokine brings receptor together
  2. JAK
  3. STAT: dimerization
  4. STAT into nucleus: gene transcription
42
Q

antigen signalling molecules

A
  • bringing dendritic cells into the required locations
  • macrophages and neutrophils upregulate phagolysosome activity and cytokine production
  • dendritic cells exhibit antigen peptides on MHC class 1 and MHC class 2
  • cytoplasmic proteosomes process An to peptides
  • dendritic cells induced to secrete cytokines