B1.1 - Microscopes And Order Of Magnitude Flashcards
Order of magnitude
- used to make approx comparisons between objects/numbers
- using powers of 10
Work out order of magnitude
-bigger number divided by smaller number
Same order of magnitude is when…
The answer is less than 10
It is one order of magnitude bigger when…
The answer is around ten (ten/above)
It is 2 orders of magnitude bigger when…
The answer is 100
Units for microscope
- 1mm = 1000 micrometers
- 1 micrometers = 1000 nanometres
Formula for total magnifications
Total magnification = eyepiece lens x objective lens
Formula for actual length / image size / magnification
Actual length =. Image size divided by magnification
Formula for scale bar
Magnification = scale bar image divided by actual scale bar length
Resolving power of a light microscope
200nm
Resolving power of scanning electron microscope
10nm
The higher the resolution….
The smaller the number
Resolving power of transmission electron microscope
0.2nm
Advantages of electron microscope
- magnify up to x 2 000 000
- high resolution
- can examine subcellular structures
Disadvantages of electron microscope
- black and white images produced
- specimens have to be dead
- expensive to buy/operate
- large/difficult to move