8.3 Photosynthesis Flashcards
What is photosynthesis?
The process by which cells synthesise organic molecules from inorganic molecules in the presence of sunlight
What does photosynthesis require?
A photosynthetic pigment
Where does photosynthesis occur in plants?
In the chloroplast
What are the two steps of photosynthesis?
Light dependent reactions
Light independent reactions
What does the light dependent reaction do?
Convert light energy from the sun into ATP
What does the light independent reaction do?
Use the chemical energy to synthesise organic compounds
Where does the light dependent reaction occur?
In the thylakoid
What are the three steps of the light dependent reaction?
Excitation of photosynthesis by light energy
Production of ATP via an electron transport chain
Reduction of NADP and the photolysis of water
What are photosystems?
Groups of photosynthetic pigments embedded within the thylakoid membrane
How are photosystems classed?
In accordance to their maximal absorption wavelengths
What happens when a photosystem absorbs light energy?
Delocalised electrons within the pigments become energised/excited
Where are excited electrons transferred to?
Carrier molecules within the thylakoid membrane
Where are excited electrons from photosystem II transferred to?
An electron transport chain within the thylakoid membrane
What do electrons lose as they are passed through the chain?
Energy
What is the energy that is lost during the electron transport chain used to do?
To translocate H+ ions into the thylakoid
What is caused by the build up of protons within the thylakoid?
An electrochemical gradient
Where do the H+ ions return to?
The stroma
How do the H+ ions return to the stroma?
Via the transmembrane enzyme ATP synthase/ chemiosmosis
What does ATP synthase use to catalyse the synthesis of ATP?
The passage of H+ ions
Where do the newly de-energised electrons from photosystem II go?
Photosystem I
What comes first photosystem I or II?
Photosystem II
What are excited electrons from photosystem I used to do?
Reduce NADP+
Where may excited electrons from photosystem I be transferred to?
A carrier molecule
What is needed in conjunction with ATP for the light independent reactions?
NADPH
What are the electrons lost from Photosystem I replaced by?
De-energised electrons from photosystem II
What replaces electrons lost from Photosystem II?
Electrons released from water via photolysis
How is water split?
By light energy
What is water split into?
H+ ions and oxygen
What triggers the release of high energy electrons?
Chlorophyll in photosystems I and II absorb light
What happens to the products of the light dependent reactions?
They are used in the light independent reactions
What are the products of the light dependent reactions?
ATP and NADPH
What is the production of ATP by the light dependent reactions called?
Photophosphorylation
What are the two types of photophosphorylation?
Cyclic or non-cyclic
What does cyclic photophosphorylation involve?
Only one photosystem
What does cyclic photophosphorylation not involve?
The reduction of NADP+
What happens to the de-energised electrons in cyclic photophosphorylation?
They return to the photosystem restoring its electron supply
In cyclic photophosphorylation, what is not needed to happen as the electron returns to the photosystem?
NADP+ is not reduced and water is not needed to replenish the electron supply
What does non-cyclic photophosphorylation involve?
Two photosystems and the reduction of NADP+
What does only non-cyclic photophosphorylation allow for?
The synthesis of organic molecules and long term energy storage
What can cyclic photophosphorylation be used to produce?
additional ATP in order to meet cell energy demands
Where does the light independent reactions occur?
In the stroma
What are the light independent reactions collectively known as?
The calvin cycle
What are the three main steps of light independent reactions?
Carbon fixation
Reduction of GP
Regeneration of RUBP
What does the calvin cycle begin with?
A 5C compound called ribulose biphosphate/RuBP
What does the enzyme Rubisco do in carbon fixation?
Catalyses the attachment of a CO2 molecule to RuBP
What is the result of the attachment of CO2 to RuBP?
An unstable 6C compound
What happens to the 6C compound?
It breaks down into two 3C compounds called glycerate-3-phosphate/GP
What does a single cycle of carbon fixation involve?
Three molecules of RuBP combining with three molecules of Co2 to make sic molecules of GP
What is GP converted into?
Triose phosphate/TP
How is GP converted into TP?
Using NADPH and ATP
What does the reduction by NADPH transfer in step 2 of the calvin cycle?
Hydrogen atoms to the compound
In step 2 of the calvin cycle, what does the hydrolysis of ATP provide?
Energy
What does each GP require?
One NADPH and one ATP to form a triose phosphate
What does a single cycle require in step ?
Six of each molecule
Of the six molecules of TP produced per cycle, what may one TP molecule be used to form?
Half a sugar molecule
What is needed to produce a single glucose monomer in the calvin cycle?
Two cycles
What happens to the remaining five TP molecules?
They are recombined to regenerate stocks of RuBP
What does the regeneration of RuBP require?
Energy derived from the hydrolysis of ATP
What are the six steps of the calvin lollipop experiment?
- Radioactive carbon-14 is added to a lollipop apparatus containing green algae
- Light is shone on the apparatus to induce photosynthesis
- The algae is killed by running into a solution of heated alcohol
- Dead algal samples are analysed using 2D chromatography
- Any radioactive carbon compounds were analysed using autoradiography
- By comparing different periods of light exposure the order of which carbon compounds are generated was determined
What can TP be used to do?
Form organic molecules or can be recombined by ATP to reform stocks of RuBP
What are chloroplasts?
The solar energy plants of a cell
What do chloroplasts do?
Convert light energy into chemical energy
What are the two types of chemical energy?
ATP or organic compounds
What possesses chloroplasts?
Only photosynthetic tissue
What are the three evidences to suggest chloroplasts were once independent prokaryotes?
- they have a double membrane structure
- they have their own DNA and ribosomes
- their metabolic processes are susceptible to certain antibiotics
What are the five structures of a chloroplast?
Thylakoids
Grana
Photosystems
Stroma
Lamellae
What are thylakoids?
Flattened disks which have a small internal volume to maximise hydrogen gradient upon proton accumulation
What are grana?
Thylakoids are arranged into stacks to increase SA:Vol ratio of the thylakoid membrane
What are photosystems (structure)?
Pigments organised into photosystems in thylakoid membrane to maximise light absorption
What is stroma?
Central cavity that contains appropriate enzymes and a suitable pH for the calvin cycle to occur
What is lamellae?
Connects and separates thylakoid stacks, maximising photosynthetic efficiency