Enzymes ORGANISATION AND THE DIGESTIVE SYSTEM Flashcards

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1
Q

Where is salivary amylase produced?

A

Salivary glands

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2
Q

Where is pepsin produced?

A

Stomach

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3
Q

Where is pancreatic amylase produced?

A

Pancreatic fluid (works in the small intestine)

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4
Q

Where is pancreatic lipase produced?

A

Pancreatic fluid (works in small intestine)

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5
Q

Where is trypsin produced?

A

Small intestine

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6
Q

Where is Maltase produced?

A

Small intestine

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7
Q

Where is peptidase produced?

A

Small intestine

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8
Q

What is salivary amylase substrate? And product?

A

Carbohydrates

Sugars

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9
Q

What is pepsins substrate? And product?

A

Proteins

Amino acids

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10
Q

What is pancreatic amylase substrate? And product?

A

Carbohydrates

Sugars

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11
Q

What is pancreatic lipase substrate? And products?

A

Lipids

Fatty acids and glycerol

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12
Q

What is trypsin substrate? And products?

A

Proteins

Amino acids

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13
Q

Why is Maltase substrate? And product?

A

Maltose

Simple sugar glucose

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14
Q

What is peptidase substrate? And product?

A

Proteins

Amino acids

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15
Q

What is salivary amylase optimum ph?

A

6.8

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16
Q

What is pepsin optimum ph?

A

1.5-1.6

17
Q

What is pancreatic amylase optimum ph?

A

6.7-7

18
Q

What is pancreatic lipase optimum ph?

A

8

19
Q

What is trypsin optimum ph?

A

7.8-8.7

20
Q

What is Maltase optimum ph?

A

6.1-6.8

21
Q

What is peptidase optimum ph?

A

4.7-6.25

22
Q

What is a complex carbohydrate?

A

Long chains of simple sugar units bonded together.

23
Q

What are carbohydrates made of?

A

Units of sugars.

24
Q

What are lipids made of?

A

Molecules of fatty acids joined to units of glycerol.

25
Q

What is a protein molecule made up of?

A

Chains of small units called amino acids.

26
Q

What do proteins do in our body?

A

Structural components of tissues such as muscles and tendons.
Hormones such as insulin.
Antibodies
Enzymes.

27
Q

How do you test for carbohydrates?

A

Iodine rest for starch - turns blue black if starch is present.
Benedicts rest for sugars - brick red when heated if sugar is present.

28
Q

How do you test for proteins?

A

Biuret test - purple of proteins is present.

29
Q

How do you test for lipids?

A

Ethanol test - cloudy white layer is a lipid is present.

30
Q

What is an enzyme?

A

Biological catalysts that speed up reactions. It is a large proteins molecule.

31
Q

What is an active site?

A

The long chains of amino acids are folded to produce a molecule with an active site. They have specific shapes so they can bind to a certain substrate molecule.

32
Q

What is the through for how enzymes work?

A

Lock and key theory. Once the substrate is in place the enzyme and the substrate bind together. The reaction takes place and the products are released form the surface of the enzyme.

33
Q

Enzymes do what to the substrate?

A

Join small molecules together or break large ones up.

34
Q

What do enzymes control?

A

Metabolism.

35
Q

What happens to an enzyme after 40 degrees?

A

The proteins structure is affected. The long amino acids begin to unravel which changes the shape of the active site. This means that substrates won’t fit in anymore. The enzyme has become denatured.

36
Q

What temperature do most human enzymes work at?

A

37 degrees.

37
Q

What effect does the PH have on enzymes?

A

The shape of an active site comes from forces between the different parts of the protein molecule. These forces hold the folded chains in place. A change in PH affects these forces. This changes the shape of the active site, which stops the enzyme form working.