4.5.1.1 Burkholderia spp

Question 1

Clinical significance for B. cepacia?

Answer 1

pneumonia in patients with cystic fibrosis or chronic granulomatous disease (similar to P. aeruginosa)
- 2nd leading cause

Question 2

B. cepacia causes in plant?

Answer 2

Onion bulb rot

Question 3

B. cepacia causes in humans?

Answer 3

foot rot

Question 4

a hereditary condition characterized by lung
congestion and infection, malabsorption of the pancreas

Answer 4

Cystic fibrosis

Question 5

Burkholderia pseudomallei
Causes ulcer, pustule, granular type of lesion
Causes systemic infection in humans

Answer 5

Melioidosis

Question 6

Other name for Melioidosis?

Answer 6

Whitmore’s disease

Aggressive granulomatous pulmonary disease

Question 7

Possible agent for bioterrorism in Bukhorlderia?

Answer 7

B. pseudomallei

Question 8

NOTE: B. gladioli is similar to cepacia and P. aeruginosa

Question 9

B. mallei MOT?

Answer 9

Ingestion of infected meat horse (Accidental host)

Question 10

Smooth, non-wrinkled and slightly raised and Dirt-like odor in BAP?

Answer 10

B. cepacia

Question 11

NOTE: Become dark pink to red after 4-7 days in MAC for NLF (B.cepacia)

Question 12

Consider as slow and weak oxidase positive?

Answer 12

B. cepacia

Question 13

(+): LDC
(-): ODC
(+): ONPG

Answer 13

B. cepacia

Question 14

3 selective media for B. cepacia

Answer 14

Pseudomonas cepacia

Oxidative fermentative polymyxin B, bacitracin, lactose (OFPBL)

Burkholderia cepacia selective agar (Most effective)

Question 15

What produces on OFPBL for B. cepacia?

Answer 15

non-fluorescing yellow/green pigment

Question 16

What is the gram stain for B. pseudomallei?

Answer 16

Bipolar staining (safety pin)

Question 17

Smooth to wrinkled colonies in BAP?

Answer 17

B. pseudomallei

Question 18

What media is use for B. pseudomallei

Answer 18

Ashdown media

Question 19

What is the result in Ashdown media?

Answer 19

After incubation for 96 hrs, wrinkled and deep pink

Question 20

What is the result in Ashdown media? B. pseudomallei

Answer 20

After incubation for 96 hrs, wrinkled and deep pink

Question 21

What is the result in Ashdown media? B. pseudomallei

Answer 21

After incubation for 96 hrs, wrinkled and deep pink

Question 22

Components of Ashdown media

Answer 22

Crystal violet
Gentamicin
Neutral red

Question 23

Odor for B. pseudomallei

Answer 23

Earthy odor

Question 24

NOTE: B. pseudomallei can ferment lactose in Ashdown media