4.5.1.1 Burkholderia spp Flashcards
Clinical significance for B. cepacia?
pneumonia in patients with cystic fibrosis or chronic granulomatous disease (similar to P. aeruginosa)
- 2nd leading cause
B. cepacia causes in plant?
Onion bulb rot
B. cepacia causes in humans?
foot rot
a hereditary condition characterized by lung
congestion and infection, malabsorption of the pancreas
Cystic fibrosis
Burkholderia pseudomallei
Causes ulcer, pustule, granular type of lesion
Causes systemic infection in humans
Melioidosis
Other name for Melioidosis?
Whitmore’s disease
Aggressive granulomatous pulmonary disease
Possible agent for bioterrorism in Bukhorlderia?
B. pseudomallei
NOTE: B. gladioli is similar to cepacia and P. aeruginosa
B. mallei MOT?
Ingestion of infected meat horse (Accidental host)
Smooth, non-wrinkled and slightly raised and Dirt-like odor in BAP?
B. cepacia
NOTE: Become dark pink to red after 4-7 days in MAC for NLF (B.cepacia)
Consider as slow and weak oxidase positive?
B. cepacia
(+): LDC
(-): ODC
(+): ONPG
B. cepacia
3 selective media for B. cepacia
Pseudomonas cepacia
Oxidative fermentative polymyxin B, bacitracin, lactose (OFPBL)
Burkholderia cepacia selective agar (Most effective)
What produces on OFPBL for B. cepacia?
non-fluorescing yellow/green pigment
What is the gram stain for B. pseudomallei?
Bipolar staining (safety pin)
Smooth to wrinkled colonies in BAP?
B. pseudomallei
What media is use for B. pseudomallei
Ashdown media
What is the result in Ashdown media?
After incubation for 96 hrs, wrinkled and deep pink
What is the result in Ashdown media? B. pseudomallei
After incubation for 96 hrs, wrinkled and deep pink
What is the result in Ashdown media? B. pseudomallei
After incubation for 96 hrs, wrinkled and deep pink
Components of Ashdown media
Crystal violet
Gentamicin
Neutral red
Odor for B. pseudomallei
Earthy odor
NOTE: B. pseudomallei can ferment lactose in Ashdown media
