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Bacte Lec [Midterms] > 3.5.5.1 Culture media and Isolation methods > Flashcards

3.5.5.1 Culture media and Isolation methods Flashcards

1
Q

Environmental conditions for solid media for AFB?

A

Incubation 35 C in the dark
5-10% CO2
Increase humidity

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2
Q

What are the 3 general types of Solid media?

A

Egg based media
Serum albumin media
Liquid media

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3
Q

Example of Egg based media?

A

Lowenstein Jensen

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4
Q

Example of Serum albumin agar media?

A

Middlebrook 7H10 / 7H11

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5
Q

Example of Liquid media?

A

BACTEC 12B medium
Middlebrook 7H9
Septi-check AFB

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6
Q

What is the inhibitory agent for Lowenstein Jensen

A

Malachite green

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7
Q

How many weeks for growth of bacteria in Middlebrook7H10/11

A

3-4 weeks growth

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8
Q

How long does it take for a growth to appear in liquid media?

A

10 days

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9
Q

What component does middlebrook 7H9 broth has?

A

Casein hydrolysate

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10
Q

Purpose of Casein hydrolysate?

A

For isoniazid resistance TB grow

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Bacte Lec [Midterms] (93 decks)

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