3.2.3.1 Lab diag for M. tuberculosis

Question 1

What are the media used for detection of M. tuberculosis?

Answer 1

Lowenstein Jensen
Middlebrook 7H10 and 7H11

Question 2

What is the appearance of colony of M. tuberculosis?

Answer 2

Raised
Dry
Rough
Nonpigmented

Question 3

What is the optimal temp for M. tuberculosis?

Answer 3

35-37

Question 4

How long does M. tuberculosis to grow in Lowenstein Jensen?

Answer 4

2-3 weeks

Question 5

How long does M. tuberculosis to grow in Middlebrook 7H10 and 7H11?

Answer 5

5-10 days

Question 6

Most commonly used solid media for M. tuberculosis?

Answer 6

Lowenstein Jensen

Question 7

Other name for Middlebrook?

Answer 7

Serum albumin agar medium

Question 8

What are the Lab diagnosis?

Answer 8

Niacin Positive
Reduce Nitrate to nitrite
Heat stable, Catalase Negative
Resistant to Nitroimidazopyran or p-nitroacetylamino-B-hydroxypropiophenone (NAP)

Resistant to Thiophene-2-carboxylic acid hydrazide (T2H) and Pyrazinamidase