3.5.4.1 Staining for Acid Fast Bacilli

Question 1

3 methods of AFB?

Answer 1

Ziehl Neelsen
Kinyoun
Auramine Rhodamine

Question 2

Acid fast stain targets what part of the Acid fast bacilli?

Answer 2

Mycolic acid

Question 3

AFB can resist?

Answer 3

3% HCl

Question 4

What color of the back ground for Ziehl Neelsen?

Answer 4

Blue

Question 5

What color of the back ground for Kinyoun?

Answer 5

Green

Question 6

What are the reagent for Kinyou?

Answer 6

Carbol fuchsin, Tergitol, 3% HCl Malachite green

Question 7

What are the reagent for Ziehl Neelsen?

Answer 7

Carbol fuchsin, Heat, HCl, Methylene blue

Question 8

What should be the size of the smear?

Answer 8

2 width by 3 length cm

Question 9

Reading on AFB stain will only be done along the solid line (in the central area) until the area with the?

Answer 9

broken line

Question 10

Method use to provide equal distribution in AFB smear

Answer 10

Coiling method

Question 11

Auramine rhodamine Flourochrome stain can be xamine at what magnification?

Answer 11

250-400 X

Question 12

Provides more sensitivity in detecting AFB?

Answer 12

Auramine Rhodamine Fluorochrome stain

Question 13

How do you report smear in AFB?

Answer 13

0 = No AFB (0)
1-2/300 fields = Repeat test
1-9 /100 fields = 1+
1-9 / 10 fields = 2+
1-9 / fields = 3+
>9/fields = 4+

Question 14

How do you report smear in Fluorochrome?

Answer 14

0 = No AFB seen (0)
1-2 / 70 fields = Repeat test
2 - 18 / 50 fields = 1+
4 - 36 / 10 fields = 2+
4- 36 / fields= 3+
>36 / fields = 4+

Question 15

If the reading is high?

Answer 15

Chance that the patient may share TB

Question 16

What is the reason why AFB may look fragmented?

Answer 16

Undergoing treatment