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Bacte Lec [Midterms] > 2.1.1 Enterobacteriaceae > Flashcards

2.1.1 Enterobacteriaceae Flashcards

1
Q

What is the gram stain for Enterobacteriaceae?

A

Gram negative

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2
Q

What is the gaseous composition of Enterobacteriaceae?

A

Facultative aerobe

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3
Q

Are all genus in Enterobacteriaceae catalase positive? if not what bacteria is catalase negative?

A

Shigella dysenteriae

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4
Q

All Enterobacteriaceae are oxidase negative except?

A

Plesiomonas shigelloides

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5
Q

Are Enterobacteriaceae glucose fermenter?

A

YES

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6
Q

Are Enterobacteriaceae reduces Nitrate to nitrite? If yes, what is the positive result?

A

Red

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7
Q

What reagent is use in Nitrate reduction?

A

Sulfanilic acid?

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8
Q

All Enterobacteriaceae are motile except?

A

Shigella
Salmonella gallinarum
Salmonella pullorum
Klebsiella
Yersinia pestis

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9
Q

Enterobacteriaceae can be seen mostly in what media?

A

BAP

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10
Q

What is the colonial morphology of Enterobacteriaceae in BAP

A

Large
Mosit
Gray

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11
Q

What is the colonial morphology of Klebsiella in BAP?

A

Capsulated (I remember it because of Klebsiella pneumoniae)

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12
Q

Klebsiella is mostly seen in what media?

A

BAP
CAP

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13
Q

What are the differential media is used in Enterobacteriaceae?

A

MacConkey Agar
Eosin Methylene Blue
Hektoen Enteric Agar
Xylose Lysine Deoxycholate

(MEHX)

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14
Q

What is the function of MacConkey agar?

A

Differentiates LACTOSE to NON LACTOSE fermenter

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15
Q

What is the positive indicator for MacConkey Agar?

A

Pink colony

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16
Q

What is the pH indicator for MacConkey agar?

A

Neutral red

17
Q

What is the component of MacConkey agar?

A

Crystal violet and Bile salt

18
Q

What is the Function of Crystal violet and bile salt?

A

Inhibits Gram positive

19
Q

What is the function of Eosin Methylene blue?

A

Identification of E. coli

20
Q

How E. coli is being identified in Eosin Methylene blue?

A

Green Metallic sheen

21
Q

What is the function of Enteric Agar?

A

Detects Lactose fermenter

22
Q

What agar is mostly used to detect Salmonella and Shigella?

A

Hektoen enteric agar
Xylose Lysine deoxycholate
Deoxycholate citrate agar
Bismuth sulfite agar

(Blackening)

23
Q

What are the Pathogenic bacteria?

A

E. coli (Diarrheagenic)
Plesiomonas shigelloides
Salmonella
Shigella
Yersinia

Bacte Lec [Midterms] (93 decks)

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