2.1.1 Enterobacteriaceae Flashcards
What is the gram stain for Enterobacteriaceae?
Gram negative
What is the gaseous composition of Enterobacteriaceae?
Facultative aerobe
Are all genus in Enterobacteriaceae catalase positive? if not what bacteria is catalase negative?
Shigella dysenteriae
All Enterobacteriaceae are oxidase negative except?
Plesiomonas shigelloides
Are Enterobacteriaceae glucose fermenter?
YES
Are Enterobacteriaceae reduces Nitrate to nitrite? If yes, what is the positive result?
Red
What reagent is use in Nitrate reduction?
Sulfanilic acid?
All Enterobacteriaceae are motile except?
Shigella
Salmonella gallinarum
Salmonella pullorum
Klebsiella
Yersinia pestis
Enterobacteriaceae can be seen mostly in what media?
BAP
What is the colonial morphology of Enterobacteriaceae in BAP
Large
Mosit
Gray
What is the colonial morphology of Klebsiella in BAP?
Capsulated (I remember it because of Klebsiella pneumoniae)
Klebsiella is mostly seen in what media?
BAP
CAP
What are the differential media is used in Enterobacteriaceae?
MacConkey Agar
Eosin Methylene Blue
Hektoen Enteric Agar
Xylose Lysine Deoxycholate
(MEHX)
What is the function of MacConkey agar?
Differentiates LACTOSE to NON LACTOSE fermenter
What is the positive indicator for MacConkey Agar?
Pink colony
What is the pH indicator for MacConkey agar?
Neutral red
What is the component of MacConkey agar?
Crystal violet and Bile salt
What is the Function of Crystal violet and bile salt?
Inhibits Gram positive
What is the function of Eosin Methylene blue?
Identification of E. coli
How E. coli is being identified in Eosin Methylene blue?
Green Metallic sheen
What is the function of Enteric Agar?
Detects Lactose fermenter
What agar is mostly used to detect Salmonella and Shigella?
Hektoen enteric agar
Xylose Lysine deoxycholate
Deoxycholate citrate agar
Bismuth sulfite agar
(Blackening)
What are the Pathogenic bacteria?
E. coli (Diarrheagenic)
Plesiomonas shigelloides
Salmonella
Shigella
Yersinia
