3.5.8.1 LAbdiag

Question 1

Detects the Niacin that convers free niacin into niacin ribonucleotide?

Answer 1

Niacin Test

Question 2

What are the reagents of niacin test?

Answer 2

10% Cyanogen Bromide
4% aniline in 96% ethanol

Question 3

Mycobacterium that are Positive in Niacin test?

Answer 3

tuberculosis
simiae
chelonae

(SiChTu)

Question 4

Mycobacterium that are negative in Niacin test?

Answer 4

avium-intracellulare
bovis

(BoA)

Question 5

2 causative agent of Pulmonary TB?

Answer 5

M. tuberculosis
M. bovis

Question 6

What are the reagents of Nitrate Reduction test?

Answer 6

HCl
Sulfanilamide
N-naphthylenediamine dihydrochloride

Question 7

What are the MB that are positive in Nitrate reduction test?

Answer 7

tuberculosis
kansasii
Szulgai
fortuitum

(KanTu ForGai)

Question 8

What are the MB that are Negative in Nitrate reduction test?

Answer 8

bovis

Question 9

Temp for heat stable catalase in catalase test?

Answer 9

68C for 20 mins

Question 10

What is the media required in catalase test?

Answer 10

Tween 80

Question 11

Reagent required in Catalase test?

Answer 11

30% H2O2

Question 12

What is the principle of Catalase test in MB?

Answer 12

Tween 80 + Mycobacteria + 30% H2O2 + Heat at 68C

Question 13

What are the bacteria produces <45mm bubbles? (NO bubble formation)

Answer 13

KanTu ForGai

kansasii
tuberculosis
fortuitum
sculgai

Question 14

What are the bacteria produces >45mm bubbles?

Answer 14

Photochromogens and Scotochromogens

Question 15

Test the ability of mycobacteria to produce lipase

Answer 15

Tween 80 hydrolysis

Question 16

What is the pH indicator for Tween 80?

Answer 16

Neutral red

Question 17

In tween 80, what bacteria is positive in 5 days? (pink to red)

Answer 17

M. kansasii
M. gordonae

Question 18

In tween 80, what bacteria is positive in 10 days? (pink to red)

Answer 18

M. tuberculosis

Question 19

What bacteria is negative in tween 80?

Answer 19

M. bovis

Question 20

Use to differentiate Rapid growers to other MB?

Answer 20

Tellurite Reduction test

Question 21

Positive indicator for Tellurite reduction?

Answer 21

Smooth, fine, Black precipitate

Question 22

Negative indicator for Tellurite reduction?

Answer 22

Gray Clumps

Question 23

Bacteria that are positive within 3 days with Arylsulfatase Test(Pink to Red)

Answer 23

fotuitum
chelonae
xenopi

(FoX cello)

Question 24

Bacteria that are positive within 14 days with Arylsulfatase Test (Pink to Red)

Answer 24

marinum
szulgai

Mari is Gai

Question 25

Bacteria that is negative in Arylsulfatase test?

Answer 25

tuberculosis

Question 26

Use to differentiate M. chelonae to other rapid growers?

Answer 26

Iron uptake

Question 27

What is the positive indicator for iron uptake?

Answer 27

Rusty brown

Question 28

Positive in iron uptake test?

Answer 28

Rapid growers

Question 29

Negative in iron uptake test?

Answer 29

M. chelonae

Question 30

Positive in Pyrazinamidase? (Red)

Answer 30

tuberculosis
marinum

(Tube ni Mari)

Question 31

Negative in Pyrazinamidase? (Colorless)

Answer 31

bovis
kansasii

Question 32

Positive in urease?

Answer 32

scrofulaceum
bovis
gastri
fortuitum

(ForGas Bov fulaceum)

Pronounce = Forgat Bob folaceum ;)

Question 33

Negative in urease?

Answer 33

gordonae
avium-intracellulare

Question 34

THIOPENE-2-CARBOXYLIC ACID HYRDAZIDE (T2H) &
PYRAZINAMIDASE, what bacteria is resistant?

Answer 34

tuberculosis

Question 35

THIOPENE-2-CARBOXYLIC ACID HYRDAZIDE (T2H) &
PYRAZINAMIDASE, what bacteria is positive?

Answer 35

bovis