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Bacte Lec [Midterms] > 3.1.1.1 Mycobacteria > Flashcards

3.1.1.1 Mycobacteria Flashcards

1
Q

General characteristics of Mycobacterium

A

Non motile
Non Sporeforming
Obligate strictly aerobe

High lipid content
Acid fast
Slow grower

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2
Q

To enhance growth of Mycobacterium, what should be added?

A

CO2 (Even though they are strictly aerobe)

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3
Q

What is the fastest growth of Mycobaterium?

A

3 days

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4
Q

Longest days to grow mycobacterum?

A

21 or 1 month days (Depending on the specie or CO2 content)

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5
Q

What is the content of lipid in Mycobacterium

A

Cord factor wax D

Mycolic acid

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6
Q

Primary stain for Acid fast?

A

Carbol fuchsin

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7
Q

Decolorizer for Carbol fuchsin?

A

Acid alcohol (3% Hydrochloric acid)

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8
Q

Counter stain for acid fast (Ziehl Neelsen)?

A

Methylene blue

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9
Q

3 methods for acid fast stain?

A

Ziehl Neelsen
Kinyoun (Cold method / Tergitol)
Auramine Rhodamine

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10
Q

How long does Mycobacterium grow in optimum temp?

A

2-6 weeks

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11
Q

What is the pH for Mycobacteria?

A

6.5 - 6.8; humid environment

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12
Q

Counter stain for acid fast (Kinyoun)?

A

Malachite green

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13
Q

What are the MT that causes TB?

A

tuberculosis
bovis
bovis BCG
africanum
caprae
canetii
microti
pinnipedii

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14
Q

What are the MT that causes Non-TB?

A

Nonphtochromogens
Photochromagens
Scotochromogens
Rapid-growers
Non cultivable

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15
Q

MT that causes Non-TB is based on what classification?

A

Runyon Classification

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16
Q

Runyon classification is based on?

A

Pigmentation production when exposed to light or dark area

17
Q

A MB non causing TB that is not based on pigmentation but rather on 3 days growth

A

Rapid growers

18
Q

Example of Non cultivable bacteria?

A

Mycobacterium leprae

19
Q

M. leprae is the causative agent for?

A

Leprosy or Ketong

20
Q

What is Non cultivable?

A

Cannot be cultured

21
Q

How do we know a bacteria is Non cultivable?

A

Inject to a mouse to see if there is manifestation

Bacte Lec [Midterms] (93 decks)

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