2nd sem: Intro to molecular medicine Flashcards
an rna blot for gene expression analysis
northern blot
is ddNTPs used in PCR
oh hell no
which enzyme is used to identify transcriptionally active regions in the DNA
Dnase1
what enzyme can destroy a phosphodiester bond
RNAsEH
vectors can transfer gene by conjugation
plasmid
this vector is use for the cloning of DNA fragments up to 5000,00 bp
YAC
this technique can detect single nucleases change based on altered mobility of the single stands of DNA
PCR-SSCP
a collection of cloned DNA fragments in which only exons are represented
cDNA libaray
this involves the use of VNTRs to establish a unique pattern of DNA of fragments for an individual
DNA fingering
which method introduces new DNA into germ cells by its injection into the somatic cells
transgenic therapy
what is the underlying principle for DNA cloning?
replication
what is the underlying principle for DNA chips/microarray technology
complementary base pairing
enzyme not required for the synthesis of cDNA
Dnase 1
in the sanger method of DNA sequencing, what causes the termination of chain elongation
the incorporation dideoxynucleotide
a plasmid is a
double stranded circular DNA
fragments of DNA can be identified by the technique of
southern bolt
a radioactive isotope labeled cDNA probe is used in
southern blotting and northern blotting
optimum temperature of DNA polymerase of T.aquaticus is
72C
the rate of migration of DNA within an agarose gel in the gel electrophoresis technique is primarily based on what factor
the size of the DNA fragments
in the insertion of a cloning vector into a cloning host, why are the host cells plated on selective medium, which usually contains an antibiotic?
because only those host cells that have been transformed by the vector will grow in the presence of the antibiotic
southern blot is used to
identify a specific DNA
application of southern blotting includes
DNA fingerprinting
northern hybridization is used to identify
specific RNA
the separation technique of charged molecules under the influence of electric current is called
gel electrophoresis
what type of probe would you use for western blot experiment
an antibody
the first step in PCR or polymerase chain reaction is
denaturation
in polymerase chain reaction, synthetic sequence of nucleotides are involved in
priming
sequence recognized and cut by a restriction endonuclease
GGATCC
when sticky ends are paired they can be joined by
DNA ligase
generating DNA fragments to produce a library of DNA fragments results from the use of
restriction endonucleases
a procedure called PCR is used to
copy gene sequences
DNA fragments and DNA probes are to southern blotting as ____ are to northern blotting
RNA fragments and DNA probes
why are heat stable DNA polymerases from thermophillic bacteria required for the polymerase chain reaction
these enzymes are stable enough to withstand temperatures required to melt DNA
in gene therapy the desired gene is introduced into a__________ using a viral vector
somatic cell
the major enzymes used in DNA recombinant technology are
restriction enzyme and DNA ligase
