Wk2 1.10-ETC

Question 1

What are the exchange rates for NADH and FADH2?

Answer 1

2.5ATP/ 1 NADH 1.5/ 1 FADH2

Question 2

About how much volume do only mitochondria take in a liver cell? Cardiac cell?

Answer 2

Liver 20% Heart 50%

Question 3

Do RBCs have mitochondria?

Answer 3

Nope

Question 4

Which mitochondrial membrane has porins?

Answer 4

The outer mitochondrial membrane

Question 5

Which ETC protein does not move H+’s across the inner mitochondrial membrane?

Answer 5

II

Question 6

Which ETC protein receives NADH?

Answer 6

I

Question 7

Which ETC protein receives FADH2?

Answer 7

II

Question 8

Which two ETC proteins surround the Q pool?

Answer 8

II and III

Question 9

What is the prosthetic group for the cytochrome ETC proteins? (III, IV)

Answer 9

Heme

Question 10

What is the prosthetic group for ETC complex I?

Answer 10

FMN, a FAD relative (but I receives NADH anyway)

Question 11

What is the prosthetic group for ETC complex II?

Answer 11

FAD

Question 12

What happens to the reduction potential as we go down the ETC? Does it become more negative or more positive?

Answer 12

it becomes more positive. The more positive the more willing it is to accept electrons. O2 at the end has a huge reduction potential!

Question 13

Whats the fancy scientific name for ETC complex I?

Answer 13

NADH-Q Oxidoreductase

Question 14

Which ETC Complex is the home to seven ion clusters?

Answer 14

Complex I

Question 15

Where do e’s get dropped off after going through the iron clusters in NADH-Q Oxidoreductase?

Answer 15

Ubiquinone (Coenzyme Q) (Q pool)

Question 16

What is the fancy scientific name for ETC Complex II? What is the electron cab that flys into this terminal again?

Answer 16

Succinate DeHydrogenase…FADH2

Question 17

What are the names for coenzyme Q when it is reduced and when it is not reduced?

Answer 17

Reduced: UbiqunOL (Its all fat happy and DRUNK with protons!!) Not reduced:Ubiquinone (both considered “coenzyme Q”)

Question 18

What two complexes reduce ubiquinone to unbiquinol? Where do they drop off the reduced Ubiquinols?

Answer 18

Complexes I and II. They drop them off at Complex III

Question 19

What is the “bottle neck” problem with the Q pool?

Answer 19

2e’s are coming in, but Cytochrome C can only carry one. so we bury one for the second half of the cycle

Question 20

What is the scientific term for ETC Complex III?

Answer 20

Cytochrome bc1 complex

Question 21

What class of molecules is characterized by an iron containing heme group bound to a protein?

Answer 21

Cytochromes

Question 22

What is the scientific name for complex IV?

Answer 22

Cytochrome Oxidase

Question 23

Which ETC complex has COPPER in it?!

Answer 23

IV

Question 24

What two metal carriers (WITH their correct charge) pick up the electrons from cytocrome c at complex IV?

Answer 24

Cu2+ and Fe3+

Question 25

What type of bond forms in between Fe and Cu in complex IV after they are reduced?

Answer 25

A peroxide bond!

Question 26

What is the enzyme that protects or tissues from free oxygen radicals? THANK YOU!

Answer 26

SUPERMAN! (SuperOxide Dismutaste)

Question 27

What is the metal in our superman enzyme that saves us from peroxide?

Answer 27

Mn (Manganese)

Question 28

What are the two molecules that take save us from H2O2?

Answer 28

Catalase and Glutathione Peroxidase

Question 29

Which step is blocked by CYANIDE and CO?

Answer 29

CytoChrome Oxidase (IV), blocks electron transport

Question 30

How many degrees of rotation are needed to release a newly made ATP from ATP Synthase?

Answer 30

120 degrees

Question 31

Which shuttle system exchanges and FADH2 for an NADH?

Answer 31

Glycerol-3-Phosphate Shuttle

Question 32

Which shuttle moves NADH into the Matrix?

Answer 32

Malate Shuttle

Question 33

Which ETC complex is the ONLY one without Fe-S as a prosthetic group?

Answer 33

IV Cytochrome Oxidase