TREMATODES (MONOECIOUS FLUKES) Flashcards
Sheep liver fluke
Fasciola hepatica
Giant Liver Fluke
Fasciola gigantica
Chinese Liver Fluke/Oriental Liver Fluke
Clonorchis sinensis
Cat Liver Fluke
Opistorchis felineus
Southeast Asian Liver Fluke
Opistorchis viverrini
Lancet fluke/Lanceolate fluke
Dicrocoelium dendriticum
➢ Large and ellipsoidal
Fasciola hepatica
➢ Thin-shelled with distinct operculum
Fasciola hepatica
➢ Yellowish to light brown in color
Fasciola hepatica
➢ Immature when laid
Fasciola hepatica
- Greater in length,
shorter cephalic cone
Fasciola gigantica
- Larger ventral sucker
Fasciola gigantica
- Reproductive organs are
located on the anterior
portion of the worm
Fasciola gigantica
- The ovum is bigger
Fasciola gigantica
The ova of F. hepatica and F. gigantica are similar although F. gigantica egg is bigger
Fasciola gigantica
➢ Yellowish-brown with distinct convex operculum
Clonorchis sinensis
➢ Resembles an “old fashion” electric bulb
Clonorchis sinensis
➢ Fully embryonated when laid
C. sinensus
Opistorchis felineus
➢ Yellowish-brown, ovoid
O. felineus
Opistorchis viverrini
➢ Measuring about 26-30 um by 15-17 um
O. felineus
Opistorchis viverrini
➢ With distinct convex operculum and a small protuberance at the abopercular end
O. felineus
Opistorchis viverrini
➢ Fully embryonated when laid
C. sinensus
Opistorchis felineus
Opistorchis viverrini
➢ Operculated and measure 35-45 μm long by 20-30 μm wide
Dicrocoelium dendriticum
➢ The eggs are thick-shelled and usually dark brown in color
Dicrocoelium dendriticum
➢ Eggs are fully embryonated when shed in feces.
Dicrocoelium dendriticum
➢ Large broad and flat body which measures about 20-50 mm in length and 6-12 mm in width
Fasciola hepatica
➢ A distinguishing feature is the cephalic cone which has a marked widening at the base of the cone (“shoulder”).
Fasciola hepatica
➢ Testes: tandem formation and highly branched
Fasciola hepatica
➢ Vitellaria: branched, found in lateral and posterior portion
Fasciola hepatica
➢ Ovary: branched/dendritic; located posterior to uterus and anterior to testes
Fasciola hepatica
➢ Uterus: short and coiled
Fasciola hepatica
adult worm is longer and its “shoulder” (cephalic cone) is less developed
Fasciola gigantica
➢ Ceca are more branched
Fasciola gigantica
➢ Branches of ovary are longer and more numerous
Fasciola gigantica
➢ Flat, transparent, elongated, rounded posteriorly and attenuated anteriorly
Clonorchis sinensis
➢ Cuticle – smooth and shiny
Clonorchis sinensis
➢ Ventral sucker – smaller than oral sucker
Clonorchis sinensis
➢ Testes – branched, arranged in tandem
Clonorchis sinensis
➢ Resembles Clonorchis sinensis
Opistorchis felineus
➢ Slightly shorter than C. sinensis measuring about 8-12 mm long and 1.5 to 3 mm wide
Opistorchis felineus
➢ Leaf-like in shape with transparent tegument
Opistorchis felineus
location of vitellaria, which are found in the middle third of the body at the level of the uterus
Opistorchis felineus
➢ Whereas the main difference is in the morphology and arrangement of testes
Opistorchis felineus
➢ Lobate testes, arranged obliquely
Opistorchis felineus
➢ Slightly similar as O. felineus
Opistorchis viverrini
adult in that the are positioned close to each other and are more deeply lobulated
Opistorchis viverrini
➢ Slender, lancet- shaped, flat
Dicrocoelium dendriticu
➢ Testes: Slightly lobulated, situated obliquely to each other anterior to the small subglobous ovary
Dicrocoelium dendriticu
➢ Oral sucker: smaller than ventral sucker
Dicrocoelium dendriticu
➢ Vitellaria: Scanty
Dicrocoelium dendriticu
1st IH:
- Water snail (Lymnea)
Fasciola hepatica
Fasciola gigantica
1st IH: Operculated snails
o Parafossarulus
o Bulimus
o Semisulcospira
o Alocinma
o Thiara
o Melanoides
Clonorchis sinensis
1st IH:
- Bithynia leachi
Opistorchis felineus
1st IH:
- Bithynia spp.
Opistorchis viverrini
1st IH: Land snails
o Cochicella
o Helicella
o Zebrina
Dicrocoelium dendriticum
2nd IH:
- Ipomea obscura (kangkong) - Nasturtium officinale (water cress)
Fasciola hepatica
Fasciola gigantica
2nd IH: Fresh water fish o Tingea tingea
o Barbus barbus
Clonorchis sinensis
2nd IH:
- Fresh water fish (Idus melanotus, Tinca tinca, Cyprinos carpio)
Opistorchis felineus
2nd IH:
- Fresh water fish
Opistorchis viverrini
2nd IH: Ants (Formica fusca)
Dicrocoelium dendriticum
METACERCARIA
Fasciola hepatica
Fasciola gigantica
Clonorchis sinensis
Opistorchis felineus
Opistorchis viverrini
Dicrocoelium dendriticum
Bile passages/bile ducts of fish- eating mammals
Clonorchis sinensis
Distal ducts occasionally pancreatic duct
Opistorchis felineus
Biliary ducts
Opistorchis viverrini
found in areas where sheep and cattle are raised and where humans consume raw watercress (Europe, Middle East and Asia)
Fasciola hepatica
more rarely in Asia, Africa, and Hawaii
Fasciola gigantica
Europe and Asia, including the former Soviet Union
Opistorchis felineus
Europe and Asia
Opistorchis viverrini
common parasite of ruminants but humans can be accidental definitive hosts
Dicrocoelium dendriticum
o Liver rot disease/Fascioliasis hepatica
Fasciola hepatica
o Acute phase: (migration of the immature fluke through the hepatic parenchyma)
Fasciola hepatica
➢ Abdominal pain, diarrhea, vomiting
Fasciola hepatica
➢ Hepatomegaly, fever, urticaria
Fasciola hepatica
➢ Eosinophilia
Fasciola hepatica
o Chronic phase: (adult fluke within the bile ducts)
Fasciola hepatica
➢ Intermittent biliary obstruction and inflammation
Fasciola hepatica
➢ Ectopic locations of infection (intestinal wall, lungs, subcutaneous tissue, and pharyngeal mucosa)
Fasciola hepatica
Diagnosis
1. Direct fecal smear examination
Fasciola hepatica
Diagnosis
2. Concentration sedimentation techniques
Fasciola hepatica
Diagnosis
3. Antibody detection
Fasciola hepatica
o Fascioliasis
Fasciola gigantica
Diagnosis
Same with F. hepatica
Fasciola gigantica
o Clonorchiasis
Clonorchis sinensis
o Carcinoma of the liver, adenocarcinoma of the gall bladder, as well as pancreatitis
Clonorchis sinensis
o Inflammation and intermittent obstruction of the biliary ducts → irritation of the bile ducts
Clonorchis sinensis
o Early infection:
➢ Leukocytosis and eosinophilia
Clonorchis sinensis
o Acute infection:
➢ Chills and fever up to 40°C
➢ Enlargement and tenderness of the liver;
Clonorchis sinensis
o Chronic infection:
➢ In the chronic stage, the clinical presentation may range from asymptomatic to mild to severe disease
Clonorchis sinensis
o Chronic infection:
➢ Cirrhosis and portal hypertension are present
Clonorchis sinensis
o Chronic infection:
➢ Cholangitis, cholelithiasis, cholangiocarcinoma, and pancreatitis
Clonorchis sinensis
Diagnosis
1. Eggs in biliary drainage (most practical diagnostic method)
Clonorchis sinensis
Diagnosis
2. Adult worms can be recovered through surgery
Clonorchis sinensis