EXPERIMENT 2, 3, 4, 5, 6, 7 POST LAB

Question 1

used for the preservation of stained fecal smears

Answer 1

Polyvinyl alcohol (PVA)

Question 2

preservation of trophozoites and cysts

Answer 2

Polyvinyl alcohol (PVA)

Question 3

*Quality control: discard if cloudy

Answer 3

Polyvinyl alcohol (PVA)

Question 4

For concentration techniques or direct fecal smear

Answer 4

5-10% Formalin

Question 5

Not suffcient for preparing permanent stained feel scr

Answer 5

5-10% Formalin

Question 6

*Quality control: Heat at 60°C before use

Answer 6

5-10% Formalin

Question 7

For bulk feces, the solution is mixed in the proportion of 9.4 ml and 0.6 ml Lugol’s for a gram of fecal material

Answer 7

Merthlolate lodine Formalin (MIF) or Thimerosal lodine Formalin (TIF)

Question 8

For fresh materials and those recovered from intestinal mucosal linings

Answer 8

Schaudinn’s fixative

Question 9

For permanent staining

Answer 9

Schaudinn’s fixative

Question 10

Enterobius vermicularis and Taenia spp.

Answer 10

CELLULOSE TAPE PREPARATION

Question 11

• provides an estimate of worm burden

Answer 11

EGG COUNT TECHNIQUE

Question 12

• for the recovery of hookworms, Ascaris, and Truchuris

Answer 12

EGG COUNT TECHNIQUE

Question 13

• 1.5 mg feces (667) = epg
• 2.0 mg feces (500) = epg

Answer 13

Direct Smear Egg Count (Method by Beaver)

Question 14

• uses 0.1 N NaOH

Answer 14

Dilution Egg Count

Question 15

• Stoll’s Egg Counting Technique

Answer 15

Dilution Egg Count

Question 16

• KTS for schistosomes

Answer 16

Thick Smear Egg Count

Question 17

• Schistosomes

Answer 17

Dilution-Fitration Egg Count

Question 18

• For accurate detection of the organisms as a supplement to other methods

Answer 18

CULTURE METHODS PURPOSES

Question 19

• For obtaining a rich yield of organisms to be used as antigen in immunologic diagnosis

Answer 19

CULTURE METHODS PURPOSES

Question 20

• For in-vitro screening of drugs

Answer 20

CULTURE METHODS PURPOSES

Question 21

• For investigating the physiology of organisms

Answer 21

CULTURE METHODS PURPOSES

Question 22

• As a source for inoculating susceptible experimental animals

Answer 22

CULTURE METHODS PURPOSES

Question 23

• for Entamoeba histolytica and some other intestinal protozoa

Answer 23

Balamuth’s Monophasic Medlum

Question 24

• Best medium

Answer 24

A. Novy, MacNeal and Nicole (NNN) Diphasic

Question 25

• For Trypanosoma cruzi and Toxoplasma

Answer 25

B. Cellular Medla

Question 26

• involves antigen-antibody reactions

Answer 26

IMMUNOLOGIC OR SEROLOGIC TEST

Question 27

• RBCs coated with antigen + test serum

Answer 27

Indirect Hemagglutination (IHA)

Question 28

• bentonite or latex or the organism

Answer 28

Flocculation or Agglutination

Question 29

parasite fixed on a slide

Answer 29

Indirect Fluorescent Antibody (IFA)

Question 30

Stained with test serum

Answer 30

Indirect Fluorescent Antibody (IFA)

Question 31

antihuman globulin with fluorescein

Answer 31

Indirect Fluorescent Antibody (IFA)

Question 32

Sandwich method

Answer 32

Indirect Fluorescent Antibody (IFA)

Question 33

Gel Diffusion (Ouchterlony) and Countercurrent Electrophoresis (CEP)
Test serum (well); adjacent (antigen); both allowed to diffuse

Question 34

Very SENSITIVE