Transcription Flashcards
___ is the synthesis of RNA from a DNA template, catalyzed by _____
Transcription, RNA polymerase
RNA polymerase copies a DNA template in the _____ direction and synthesizes a single-stranded RNA molecule in the ___ direction
copy DNA template: 3’ to 5’ direction
synthesizes single-stranded RNA: 5’ to 3’ direction
____ are segment within the DNA that do not code for protein (are removed) while ____ are segments that produce mature RNA (are spliced together)
Introns - are removed
Exons - are spliced
Translation happens in the ____ (nucleus, cytoplasm)
cytoplasm
A primer is not required in RNA polymerase since it can initiate synthesis of new chains/ Yes or No?
Yes!
The RNA polymerase binds to a promoter region that contain the consensus sequence ____ which is located ____ bases ____ (upstream/downstream) fromt he start of transcription
TATAAT or the Pribnow or TATA box
10 base pairs UPSTREAM
____ (cite the sequence) is a second consensus sequence located about 35 nucleotides upstream from the pribnow box.
TTGACA
____ is produced as a polycistronic transcript that is translated as it is being transcrbed
mRNA
____ is produced as a large transcript that is cleaved. e.g. 70s ribose (30x and 50s)
rRNA
In mRNA synthesis, the ____ contains TATATAA located ___ base paires ___ (upstream, downstream)
TATA (hogness box)
25 base pairs upstream
in mRNA synthesis, the ___ box is found about 70 base pairs upstream from the start site.
CAAT box
Again, the cap is added at the ___ end while the ___ is added to the 3’ end
Cap at 5
Poly A at 3
____ is produced by the RNA polymerase III which contains both introns and exons. Exons are mature RNA while introns are removed
heterogenous nuclear RNA
____ serves as the precursor in processing hnRNA which yields mature mRNA that enters the cytoplasm through nuclear poses
ATP yes ATP!
No template is required
____ is the enzyme that produces tRNA
RNA polymerase III
