Chromatorgraphy and Membrane and Transport Flashcards
____ cleaves within short, specific sequences of DNA, which can be used to obtain DNA fragments for study or for insertion into the DNA from other sources
Restriction enzymes
___ separates DNA fragments in size
Gel Electrophoresis
Southern blots are produced when ___ probe hybridizes with ____ on a nitrocellulose blot of a gel.
DNA, DNA
“DD in the south”
Northern blots are produced when ___ probe hybridizes with ____ on a nitrocellulose blot of a gel.
DNA-RNA
“DaRNA up north”
____ is a technique where proteins are separated by gel electrophoresis and probed with antibodies that bind a specifc protein
Western blot
___ is the distance travelled by the individual component divided by the total distance traveled by the solvent
Retention factor
What membrane transporter is inhibited by Digoxin?
NaK ATPase (antiporter)
Cardiotonic steroids = Cardiac Glycosides
What membrane transporter is defective in Nephrogenic DI?
aquaporin
mutated ADH receptor in kidney
[Diagnose]
unique facial features, recurrent infections, leukocytosis, decrease neutrophil chemotaxis, growth and mental retardation
what is deficient?
GDP-Fucose transporter deficiency
[Diagnose]
Neuropsychiatric symptoms
CLD, parkinsonian tremors, decreased facial expression, choreoathetosis
what is deficient?
ATP7B = Wilsons disease
ATP7A = Menkes = kinky = kinkA
Menkes = wiry brittle hair
Ouabain inhibits what transporter?
Na/K ATPase
What is an important protein in MOA of digoxin on myocytes?
Na/Ca exchange protein(NCX)
What is the confirmatory test for cystic fibrosis?
increased NaCl in sweat
What is the primary determinant of RMP in cardiac pacemaker?
Background K conductance
___ cell junction that makes cells impermeable
Tight Junction (Zona Occludens)
