Glycolysis

Question 1

____ pathway which converts glucose to pyruvate

Answer 1

Glycolysis

Question 2

Hexokinase: muscles :: _____ : liver

Answer 2

glucokinase

not inhibited by its product G6P

Question 3

The first committed step in glycolysis ____. Also, name the enzyme.

Answer 3

phosphorylation of F6P to F1,6,P. PFK1

Question 4

The net result of glycolysis (include product and ATP used)

Answer 4

2 pyruvate, use of 2 ATP

Question 5

The active site in the oxidation of G3P to 1,3 BPG contains ___ (amino acid)

Answer 5

Cysteine

Question 6

This enzyme drives formation of pyruvate from PEP. Also very active in the fed state.

Answer 6

Pyruvate kinase

Question 7

In the RBC, 1,3 BPG can be converted to 2,3 BPG. Increase in its concentration can ____ (increase, decrease) the affinity of Hb to O2

Answer 7

decrease

Question 8

In terms of Km for glucose, hexokinase ___ glucokinase (greater, lesser, same)

Answer 8

Km: hexokinase is lesser than glucokinase

Affinity: hexokinase is greater than glucokinase

Question 9

The two major regulatory enzymes in the skeletal muscle

Answer 9

Hexokinase and PFK1

Question 10

The two major regulatory enzyme in the liver

Answer 10

Glucokinase, PFK 1 activated by F2,6P, and pyruvate kinase

Question 11

Pyruvate kinase is activated by ____ in the fed state and inhibited by _____ in the fasting state.

Answer 11

Activated by: F1,6BP

Inbibited by alanine and phosphorylation in the liver

Question 12

What are the fates of pyruvate during hypoxia and alcohol ingestion

Answer 12

lactate formation (lactate dehydrogenase)

Question 13

Aside from lactate formation, pyruvate can be converted to ____ and ____

Answer 13

acetyl-CoA (pyruvate dehydrogenase) and Oxaloacetate (pyruvate carboxylase)

Question 14

The cytosolic DHAP is reduced to G3P by NADH in this shuttle system

Answer 14

Glycerol Phosphate Shunt

1 NADH = 1.5 ATP