TRANSCRIPTION Flashcards

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1
Q

3 parts to the central dogma- All cells…

A
  1. store genetic code information in DNA
  2. use transcription
  3. use translation
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2
Q

Transcription

A

Transfer the genetic code information in DNA to an RNA molecule called a messenger RNA

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3
Q

Translation

A

Convert the genetic code information in the messenger RNA into the protein the cell needs

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4
Q

The genetic code consists of the

A

Genetic alphabet (A, C, G, T) and the genetic code words (codons)
Language of life

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5
Q

All species use the genetic code/set of rules to

A
  1. store genetic instructions in DNA
  2. use these instructions to make all the proteins they require
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6
Q

The linear sequence of codons, also called triplets, in the gene’s coding region determines….

A

Determines the linear sequence of amino acids in the protein the gene encodes

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7
Q

Each codon in the gene consists of

A

Three consecutive nucleotides that correspond to one of the 20 types of amino acids

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8
Q

What are the codons

A

The 64 “genetic code words” that form nature’s remarkable genetic langauge

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9
Q

Nature arranges the four nucleotides (A, C, G, T) that form DNA into all possible combinations to form

A

64 genetic code words

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10
Q

The genetic code is _______ among all life

A

Universal

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11
Q

Cells use ________ to begin accessing the genetic instructions in a gene to make the protein the gene encodes

A

Transcription

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12
Q

The cell must ______ (______) the gene when it needs the protein the gene encodes

A

Express (activate)

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13
Q

Transcription

A

Makes an RNA copy of the gene called a messenger RNA (mRNA) or transcript

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14
Q

mRNA

A

Is a copy of the gene’s coding strand
It contains the nucleotide sequence that determines the amino acid sequence of the protein the gene encodes

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15
Q

RNA polymerase synthesizes

A

the messenger RNA molecule during transcription

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16
Q

RNA Polymerase reads the

A

nucleotide sequence of the gene’s non-coding (template) strand
-it follows the rules of complementary base pairing when linking the ribonucleotides together
-Proof reading ability

17
Q

The nucleotide uracil replaces _____ in mRNA

A

Thymine

18
Q

RNA polymerase IV and V

A

Makes small RNAs in plants

19
Q

How many phases in transcription

A

3

20
Q

Initiation phase

A

Begins when the cell receives the signal to express (activate) the gene
-The transcription initiation complex is assembled, positioned, and activated at the promoter
-The complex is formed from the general transcription factors and RNA polymerase binding to each other and to the promoter

21
Q

Elongation phase

A

RNA polymerase synthesizes messenger RNA at the transcription start site
-RNA polymerase bonds the ribonucleotides together to make mRNA

22
Q

Termination phase

A

Occurs when RNA polymerase reaches the transcription stop site sequence
-RNA polymerase pauses and stops mRNA synthesis
-RNA polymerase moves ~10-35 nucleotides past the transcription stop site
-RNA polymerase releases the mRNA molecule

23
Q

Termination phase is also called

A

Poly-Adenylation signal
Termination sequence