STERILIZATION AND DISINFECTION Flashcards
memorization
Total absence of viable microorganisms as assessed by no growth on any medium
Sterility
Kills bacteria
Bactericidal
Inhibits growth of bacteria
Bacteriostatic
Removal or killing of all microorganisms; process to achieve sterility
Sterilization
Removal or killing of disease-causing microorganisms (inanimate objects)
Disinfection
Any procedure that inhibits the growth and multiplication of microorganisms
Antisepsis
mechanism of moist heat sterilization/disinfection:
coagulates proteins
100C for 15-30 minutes; kills all vegetative organisms but not all spores or viruses
Boiling
Alternate heating (kills vegetative cells), incubation (spores germinate), heating (kills remaining vegetative cells)
Fractionation
Fractionation method; Flowing steam for 30 minutes on 3 successive days
Tyndallization
Fractionation method; 75-80C for 2 hours on 3 successive days
Inspissation
Uses steam under pressure; this is the most effective method of sterilization; kills all organisms including viruses and spores
Autoclaving
Autoclaving process:
121C at 15 pounds pressure for 15-30 minutes
In autoclaving, pressure is used to ________.
raise the temperature
what kills the bacteria in autoclaving?
temperature