STERILIZATION AND DISINFECTION Flashcards

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1
Q

Total absence of viable microorganisms as assessed by no growth on any medium

A

Sterility

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2
Q

Kills bacteria

A

Bactericidal

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3
Q

Inhibits growth of bacteria

A

Bacteriostatic

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4
Q

Removal or killing of all microorganisms; process to achieve sterility

A

Sterilization

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5
Q

Removal or killing of disease-causing microorganisms (inanimate objects)

A

Disinfection

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6
Q

Any procedure that inhibits the growth and multiplication of microorganisms

A

Antisepsis

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7
Q

mechanism of moist heat sterilization/disinfection:

A

coagulates proteins

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8
Q

100C for 15-30 minutes; kills all vegetative organisms but not all spores or viruses

A

Boiling

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9
Q

Alternate heating (kills vegetative cells), incubation (spores germinate), heating (kills remaining vegetative cells)

A

Fractionation

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10
Q

Fractionation method; Flowing steam for 30 minutes on 3 successive days

A

Tyndallization

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11
Q

Fractionation method; 75-80C for 2 hours on 3 successive days

A

Inspissation

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12
Q

Uses steam under pressure; this is the most effective method of sterilization; kills all organisms including viruses and spores

A

Autoclaving

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13
Q

Autoclaving process:

A

121C at 15 pounds pressure for 15-30 minutes

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14
Q

In autoclaving, pressure is used to ________.

A

raise the temperature

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15
Q

what kills the bacteria in autoclaving?

A

temperature

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16
Q

Quality control of autoclaving:

A

spores of Bacillus stearrothermophilus incubated at 56C

17
Q

Recommended for milk and dairy products

A

Pasteurization

18
Q

Pasteurization batch:

A

Low-temperature holding (63 deg. celsius for 30 minutes)

19
Q

Pasteurization Flash:

A

High-temperature short time at 72 deg. celsius for 15 seconds

20
Q

Kills microorganisms by oxidation

A

Dry heat

21
Q

Oven temperature and duration for killing microorganisms:

A

160 - 180 deg. celsius for 1-2 hours

22
Q

Used for materials that cannot be autoclaved (rubber goods, plastic tubes, etc.); Strict requirements of concentration, humidity

A

Ethylene oxide gas (cold sterilization)

23
Q

Quality control for cold sterilization:

A

spores of Bacillus subtilis incubated at 35-37C

24
Q

Destroys vegetative forms on inanimate objects (microbicidal)

A

Disinfectant

25
Q

A disinfectant that is safe for delicate lensed instruments; usually poor activity against Pseudomonas

A

2% aqueous glutaraldehyde

26
Q

A diphenyl cationic analog that is a useful topical disinfectant; can also be antiseptic

A

Chlorhexidine

27
Q

Endpoint of phenol coefficient:

A

The lowest concentration that kills test organisms in 10 minutes at 20C

28
Q

test organisms of phenol coefficient:

A

Salmonella typhi
Staphylococcus aureus

29
Q

agent used on living tissue:

A

Antiseptic

30
Q

Why is 70% alcohol more effective than 95%?

A

because water is necessary for protein denaturation

31
Q

major form of alcohol used in hospitals:

A

Isopropyl alcohol

32
Q

Mixture of tincture:

A

alcohol + iodine

33
Q

Mixture of iodophor:

A

detergent + iodine

34
Q

BIOLOGIC INDICATORS:

Autoclave:

A

Bacillus stearothermophilus

35
Q

BIOLOGIC INDICATORS:

Ionizing radiation:

A

Bacillus pumilus

36
Q

BIOLOGIC INDICATORS:

Dry heat oven:

A

Bacillus subtilis var niger

37
Q

BIOLOGIC INDICATORS:

Ethylene oxide:

A

Bacillus subtilis var globigii