MISCELLANEOUS BACTERIA (Atypical microorganisms) Flashcards
know the bacteria, know the disease
Causes primary atypical pneumonia and has been associated with joint and other infections/ walking pneumonia:
Mycoplasma pneumoniae
Causes postpartum fever and has been found with other bacteria in uterine tube infections:
Mycoplasma hominis
Cause of niongonococcal urethritis in men and is associated with lung disease in premature infants of low birth weight:
Ureaplasma urealyticum
Closely related to M. pneumoniae and has been associated with urethral and other urogenital infections.
Mycoplasma genitalium
In PPLO agar (pleuropneumonia-like organisms) M. hominis characteristic colony appearance:
“Fried egg” colonies
stain used for the isolated colony of Mycoplasma in agar:
Dienes stain
- Bacterial colonies lose the stain in 15 minutes
- Mycoplasma colonies retain blue color
Obligate intracellular gram-negative bacteria that lack mechanisms for the production of metabolic energy and cannot synthesize ATP; cell wall lacks peptidoglycan; do not gram stain well:
Chlamydia and Chlamydophilia
Only Chlamydia spp. susceptible to Sulfonamide:
Chlamydia trachomatis
C. trachomatis serotype that causes trachoma and blindness (found in Africa)
Serotype A, B, C
C. trachomatis serotype that causes Urethritis, Pelvic Inflammatory Disease (PID), Neonatal pneumonia, Neonatal conjunctivitis (Ophthalma neonatorum)
Serotypes D through K
C. trachomatis serotype that causes Lymphogranuloma venereum (LGV)
L1, L2, L3
Chlamydia spp. that causes pneumonia, bronchitis, pharyngitis, sinusitis?
C. pneumoniae
Chlamydia spp. that causes parrot fever, pneumonia, fever of unexplained origin
C. psittaci
Infectious form of Chlamydia; enters cell via endocytosis:
Elementary bodies
Reproductive form of Chlamydia; replicates in cells by binary fission:
Reticulate bodies
EBs in Giemsa stain =
Stains purple
RBs in Giemsa stain =
Stains blue
C. trachomatis in Dilute Lugol’s iodine =
C. trachomatis inclusion appear brown because of glycogen
Chlamydia spp that has glycogen as inclusions:
C. trachomatis
Cell culture for Chlamydia:
McCoy cells treated with cycloheximide
Molecular methods for Chlamydia:
Nucleic acid amplification testing (NAAT)
Chlamydia is often found as coinfection with _________.
N. gonorrhoeae
Bacterium that causes tranch fever, can be cultivated in cell-free medium.
Rochalimea quintana/ Bartonella quintana/ Ricketssia quintana
Agglutinating antibodies produced react against antigens of proteus strains:
Weil-Felix Reaction
