ROUTINE PRIMARY MEDIA FOR AEROBES AND FACULTATIVE ANAEROBES Flashcards
memorization
Enriched medium that will grow most non fastidious bacteria.
Blood agar (sheep)
Blood agar contains:
Tryptic soy agar with 5% sheep blood added. Allows differentiation of hemolysis.
Enriched medium for Haemophilus and Neisseria. Supplies X and V factors. Incubate in increased CO2
Chocolate agar
Selective medium for gram-positive microorganisms
Columbia colistin nalidixic acid agar (CNA)
Actions of colistin and nalidixic acid in CNA
Colistin and nalidixic acid suppress most Gram-negative bacteria.
Selective medium for gram-positive cocci and anaerobic gram-negative rods
Phenylethyl alcohol agar (PEA)
Selective medium for S. pyogenes and S. agalactiae
Streptococcal selective agar (SSA)
Selective differential medium for isolation of enteric gram-negative rods (Enterobacteriaceae)
Eosin-Methylene Blue (EMB)
MacConkey agar (MAC)
The action of Eosin and methylene blue in EMB agar:
Eosin and methylene blue inhibit GRAM-POSITIVES
Lactose fermenters in EMB:
green-black or purple colonies
E. coli in EMB:
E. coli produces green metallic sheen
Non-lactose fermenters in EMB:
Colorless
A component in MAC that inhibit most gram-positives
Bile salts and crystal violet
Lactose fermenters in MAC:
lactose fermenters in MAC are PINK
Non-lactose fermenters in MAC:
Colorless