ROUTINE PRIMARY MEDIA FOR AEROBES AND FACULTATIVE ANAEROBES Flashcards

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1
Q

Enriched medium that will grow most non fastidious bacteria.

A

Blood agar (sheep)

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2
Q

Blood agar contains:

A

Tryptic soy agar with 5% sheep blood added. Allows differentiation of hemolysis.

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3
Q

Enriched medium for Haemophilus and Neisseria. Supplies X and V factors. Incubate in increased CO2

A

Chocolate agar

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4
Q

Selective medium for gram-positive microorganisms

A

Columbia colistin nalidixic acid agar (CNA)

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5
Q

Actions of colistin and nalidixic acid in CNA

A

Colistin and nalidixic acid suppress most Gram-negative bacteria.

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6
Q

Selective medium for gram-positive cocci and anaerobic gram-negative rods

A

Phenylethyl alcohol agar (PEA)

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7
Q

Selective medium for S. pyogenes and S. agalactiae

A

Streptococcal selective agar (SSA)

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8
Q

Selective differential medium for isolation of enteric gram-negative rods (Enterobacteriaceae)

A

Eosin-Methylene Blue (EMB)
MacConkey agar (MAC)

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9
Q

The action of Eosin and methylene blue in EMB agar:

A

Eosin and methylene blue inhibit GRAM-POSITIVES

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10
Q

Lactose fermenters in EMB:

A

green-black or purple colonies

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11
Q

E. coli in EMB:

A

E. coli produces green metallic sheen

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12
Q

Non-lactose fermenters in EMB:

A

Colorless

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13
Q

A component in MAC that inhibit most gram-positives

A

Bile salts and crystal violet

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14
Q

Lactose fermenters in MAC:

A

lactose fermenters in MAC are PINK

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15
Q

Non-lactose fermenters in MAC:

A

Colorless

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16
Q

Selective medium for E. coli O157-H7

A

MacConkey sorbitol agar (SMAC)

17
Q

E. coli O157-H7 in SMAC:

A

E. coli O157-H7 does not ferment sorbitol.
colonies are colorless

18
Q

Inhibitors present in Hektoen Enteric Agar (HE) that inhibit normal GI flora

A

Bile salts
Bromthymolblue
Acid fuchsin

19
Q

Nonpathogens in HE are:

A

Orange to salmon pink

20
Q

Non-lactose fermenters in HE:

A

Green to blue-green

21
Q

H2S positive colonies in HE:

A

Have BLACK PRECIPITATE

Salmonella is H2S positive
In HE, Salmonella is green to blue-green (non-lactose fermenter) colored colonies with black precipitate

22
Q

Selective, differential medium for isolation of Salmonella and Shigella in stool

A

Xylose Lysin Deoxycholate (XLD)

23
Q

Inhibitor present in XLD

A

Deoxycholate

Deoxycholate inhibits many gram-negative rods and gram-positives

24
Q

Yellow colonies in XLD:

A

E. coli

25
Q

Yellow with black centers in XLD:

A

Some proteus spp.

26
Q

Colorless or red colonies in XLD:

A

Shigella

27
Q

Red colonies with black centers in XLD:

A

Salmonella (H2S positive+)

28
Q

Selective medium for Salmonella and Shigella

A

Salmonella-Shigella Agar (SSA)

29
Q

Inhibitors present in SSA (Salmonella-Shigella Agar) that inhibits coliforms:

A

Brilliant green
Bile salts

30
Q

Colonies of Salmonella and Shigella in SSA:

A

Salmonella and Shigella DO NOT FERMENT LACTOSE - Colonies are colorless

Salmonella is H2S positive - Black center

31
Q

Selective enrichment medium for isolation of Salmonella and Shigella from stools and rectal swabs

A

Gram-negative broth (GN)

32
Q

Enrichment broth used for recovery of Salmonella from stool:

A

Selenite broth
Tetrathionate broth

33
Q

Selective enrichment medium for isolation of Campylobacter from stool:

A

Campylobacter Blood Agar (Campy BAP)

34
Q

Incubation requirements of Campy BAP:

A

Incubate plates in increased CO2 at 42 deg. celsius
(5% O2; 10% CO2)

35
Q

Organisms that grow at 42 deg. celsius:

A
  1. Campylobacter
  2. P. aeruginosa