BACTERIAL GROWTH AND REPRODUCTION Flashcards

memorization

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1
Q

Bacteria reproduce by asexual reproduction via _________.

A

transverse binary fission

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2
Q

Growth cycle

Little or no multiplication but enzymes are very active. A period of adjustment and adaption.

A

Lag phase

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3
Q

Growth cycle

Organisms grow at maximum rate (exponential rate). Most sensitive to antimicrobials.

A

Logarithmic/ exponential phase

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3
Q

Growth cycle

Plateau; growth ceases because nutrients are exhausted or toxic metabolic products have accumulated

A

Stationary phase

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4
Q

Groth cycle

Direct microscopic count may retain constant but variable count slowly decreases.

A

Decline of death

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5
Q

Direct uptake DNA from surrounding environment; Allows for evaluation of DNA over time; Very useful in micro labs; introduces genes to bacteria for replication

A

Transformation

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6
Q

Transfer from one cell to another via pilus; requires physical contact of two organisms; DNA transferred via plasmids

A

Conjugation

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7
Q

Are small DNA molecule within a cell:

A

Plasmids

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8
Q

Transfer of DNA via a bacteriophage (virus that infects bacteria); virus picks up DNA, transfers to another bacteria

A

Transduction

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9
Q

Nuclear material enters bacteria; Multiplies, lyses cell; Releases progeny viruses

A

Lytic cycle

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10
Q

Nuclear material enters cell; Incorporates in host DNA; May later become excised then enter lytic phase’ Genes for some bacterial toxins are transferred to non-toxic strains via lysogeny

A

Lysogenic cycle

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11
Q

Also known as “Jumping genes”. DNA segment within bacterial DNA;

A

Transposons

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12
Q

A mechanism of transfer of resistance to antibiotics; can even transfer antibiotic resistance genes between two different bacterial species

A

Transposition

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13
Q

Requires preformed organic compounds (e.g. sugar, amino acids) for growth. Includes most medical important bacteria

A

Heterotrophs/Organotrophs

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14
Q

Do not require preformed organic compounds for growth because they can synthesize the from inorganic compounds and carbon dioxide

A

Autotrophs/Lithotrophs

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15
Q

Use light as energy source

A

Phototrophs

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16
Q

Use redox potential generated by various chemical reactions

A

Chemotrophs

17
Q

Grows in the presence of atmospheric (free) oxygen

A

Aerobe

18
Q

Cannot grow in absence of free oxygen, which serves as final electron acceptor.

A

Obligate Aerobe

19
Q

Obligate aerobes contain ____________, which protects from the toxic oxygen

A

superoxide dismutase

20
Q

Requires reduced amount of free oxygen.

A

Microaerophile

21
Q

Grows in the absence of atmospheric (free) oxygen

A

Anaerobe

22
Q

Cannot grow in the presence of atmospheric (free) oxygen; lack superoxide dismutase, catalase, and cytochromic-C oxidase

A

Obligate anaerobe

23
Q

obligate anaerobe uses _____, _____ and _____ as final electron acceptor

A

Nitrates, sulfates, and carbonates

24
Q

Fundamentally an aerobe but can grow in the absence of atmospheric (free) oxygen

A

Facultative anaerobe

25
Q

Does not grow well, but survives in the presence of atmospheric (free) oxygen

A

Aerotolerant

26
Q

Mention Obligate Aerobes:

A
  • Pseudomonas aeruginosa
  • Nocardia
  • Bacillus
  • Mycobacterium
  • Corynebacterium

Remember: “May Cash sa PNB” / MC PNB

27
Q

Mention Obligate Anaerobes:

A
  • Actinomyces
  • Bacteroides
  • Clostridium
  • Fusobacterium
  • Eubacterium
  • Prevotella

Remember: ABC FEP

28
Q

Mention Aerotolerant microorganisms:

A
  • Lactobacillus
  • Propionibacterium
29
Q

Mention Microaerophiles:

A
  • Campylobacter
  • Helicobacter

Remember: both ends with “-bacter”

Campylobacter requires 5% O2 for incubation

30
Q

Difference and similarity of Bacillus and Clostridium:”

A

Difference:
Bacillus - Obligate aerobe
Clostridium - Obligate anaerobe

Similarity: Both are spore forming

31
Q

Difference and similarity of Nocardia and Actinomyces

A

Difference:
Nocardia - Obligate aerobe, ACID-FAST (+)
Actinomyces - Obligate anaerobe, NON ACID-FAST

Similarity: both are fungi-like/filamentous/branching bacteria

32
Q

Growth is enhanced by the increased CO2; independent from oxygen requirement.

A

Capnophile

33
Q

Capnophilic incubation:

A

5-10% CO2

34
Q

Mention Capnophilic organisms:

A
  • Streptococcus
  • Campylobacter
  • Haemophilus
  • Helicobacter
  • Moraxella catarrhalis
  • Mycobacterium
  • Pathogenic Neisseria

remember: “SCHHMMP”

35
Q

grow below 10C:

A

Psychrophilic

36
Q

examples of psychrophilic organisms:

A

Listeria monocytogenes
Yersinia enterocolitica (blood bank contaminant [pRBC])

37
Q

grow at 20 - 40C (best at 30-37C). Most pathogens

A

Mesophilic

38
Q

grow at 50-55C (basis of test for effective autoclaving)

A

Thermophilic

39
Q

example of an acidophile organism:

A

Lactobacillus

40
Q

example of an Alkalophiles/basophile microogranusm:

A

Vibrio

41
Q

Enrichment medium for vibrio:

A

Alkaline peptone water