GRAM-NEGATIVE COCCOBACILLI Flashcards

memorization

1
Q

Bacteria included in the gram-negative coccobacilli classification:

A
  1. Haemophilus
  2. Bordetella
  3. Legionella
  4. Gardnerella
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2
Q

H. influenzae is also known as:

A

Pfeiffer’s bacillus

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3
Q

How many serological types does H. influenzae have that have polysaccharide capsules?

A

6 serological types (a, b, c, d, e, f)

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4
Q

Most common H. influenzae serological type that causes meningitis in infants under 2 years of age in the U.S

A

Type b

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5
Q

Type b causes:

A
  • epiglotittis (“cherry red’ epiglotittis)
  • pneumonia
  • cellulitis
  • otitis media
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6
Q

H. influenzae can produce IgA protease

True or False:

A

True

mnemonic: ‘SHiNeNe”
- S. pneumoniae
- H. influenzae
- Neisseria gonorrhoeae
- Neisseria meningitidis

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7
Q

H. aegyptius (now part of Haemophilus) is also known as:

A

Koch-Weeks bacillus

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8
Q

H. aegyptius causes “_______” conjunctivitis

A

“pink eye” conjunctivitis

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9
Q

Haemphilus species that are considered normal flora in the upper respiratory tract:

A
  1. H. haemolyticus
  2. H. parahemolyticus
  3. H. parainfluenzae; others
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10
Q

Cause of ulcerative chancroid, an STD seen as a painful genital ulcer:

A

H. ducreyi

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11
Q

Cause of ulcerative chancre, an STD seen as a painless genital ulcer:

A

Primary syphilis

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12
Q

H. ducreyi in gram stain is described as “____________________” appearance

A

H. ducreyi in gram stain:

“school of fish” appearance

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13
Q

Colony appearance of Haemophilus influenzae:

A

Dew-drop colonies

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14
Q

Haemophilus species require ____ and/or _____ factors

A

X and/or V factors

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15
Q

Agar that supplies both X and V factors:

A

Chocolate agar

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16
Q

A phenomenon where Haemophilus colonies grow more luxuriously next to Staphylococcus streak:

A

“Satellite phenomenon”

17
Q

Haemophilus requirements:

H. influenzae:
- X factor (?)
- V factor (?)
- Hemolysis (?)
- ALA (?)

A

H. influenzae:
- X factor (+)
- V factor (+)
- Hemolysis (-)
- ALA (-)

18
Q

Haemophilus requirements:

H. aegyptius:
- X factor (?)
- V factor (?)
- Hemolysis (?)
- ALA (?)

A

H. aegyptius:
- X factor (+)
- V factor (+)
- Hemolysis (-)
- ALA (-)

19
Q

Haemophilus requirements:

H. haemolyticus:
- X factor (?)
- V factor (?)
- Hemolysis (?)
- ALA (?)

A

H. haemolyticus:
- X factor (+)
- V factor (+)
- Hemolysis (+)
- ALA (-)

20
Q

Haemophilus requirements:

H. parahaemolyticus:
- X factor (?)
- V factor (?)
- Hemolysis (?)
- ALA (?)

A

H. parahaemolyticus:
- X factor (-)
- V factor (+)
- Hemolysis (+)
- ALA (+)

21
Q

Haemophilus requirements:

H. parainfluenzae
- X factor (?)
- V factor (?)
- Hemolysis (?)
- ALA (?)

A

H. parainfluenzae:
- X factor (-)
- V factor (+)
- Hemolysis (-)
- ALA (+)

22
Q

Haemophilus requirements

H. paraphrophilus
- X factor (?)
- V factor (?)
- Hemolysis (?)
- ALA (?)

A

H. paraphrophilus:
- X factor (-)
- V factor (+)
- Hemolysis (-)
- ALA (+)

23
Q

Haemophilus requirements

H. ducreyi:
- X factor (?)
- V factor (?)
- Hemolysis (?)
- ALA (?)

A

H. ducreyi:
- X factor (+)
- V factor (-)
- Hemolysis (Wk+)
- ALA (-)

24
Q

Haemophilus requirements:

H. aphrophilus:
- X factor (?)
- V factor (?)
- Hemolysis (?)
- ALA (?)

A

H. aphrophilus:
- X factor (-)
- V factor (-)
- Hemolysis (-)
- ALA (+)

25
Causative agent of the whooping cough; "100 day cough"
Bordetella pertussis
26
toxin produced by B. pertussis which impairs recruitment of neutrophils
Pertussis toxin
27
In B. pertussis infection, instead of neutrophilia, what leukocyte increases?
Lymphocytes (Lymphocytosis)
28
Stages of pertussis:
- Catarrhal - Paroxysmal - Convalascent
29
Most contagious pertussis stage:
Catarrhal
30
Recovery stage of pertussis:
Convalascent
31
Stage of pertussis where "whooping cough" is observed:
Paroxysmal
32
What differentiates B. pertussis from other Bordetella species?
B. pertussis - urease (-) - nitrate (-) - non-motile - oxidase (-) B. parapertusis - urease (+ after 18 hours) B. bronchisepta - Urease (+) - nitrate (+) - motile - oxidase 9+)
33
Fastidious, gram-negative rod but does not gram stain well,; silver stains used (e.g. Dieterie stain)
Legionella
34
best medium for Legionella:
Buffered Charcoal Yeast Extract Agar (BCYE)
35
Legionella requires _____ and _______ for growth:
iron and L-cysteine
36
Diseases caused by Legionella pneumophilia:
- Legionnaire's disease - Pontiac fever
37
Bacteria associated with Bacterial Vaginosis; tiny fastidious rod:
Gardnerella vaginalis (formerly Haemophilus vaginalis)
38
Culture media for G. vaginalis:
- V (vaginalis) agar - HBT (human blood tween) agar (best medium for G. vaginalis)
39
Vaginal epithelial cells covered with bacteria:
Clue cells