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CLINICAL MICROBIOLOGY - BACTERIOLOGY (TopnotcherQT) > BIOCHEMICAL TESTS FOR IDENTIFICATION OF ENTEROBACTERIACEAE > Flashcards

BIOCHEMICAL TESTS FOR IDENTIFICATION OF ENTEROBACTERIACEAE Flashcards

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1
Q

Good test to differentiate Enterobacteriaceae from non-fermenters

A

Cytochrome oxidase test

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2
Q

Cytochrome oxidase positive result:

A

Maroon-violet color within 30 seconds

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3
Q

Enumerate cytochrome oxidase-positive organisms:

A
  • Campylobacter
  • Aeromonas
  • Neisseria
  • Pseudomonas
  • Pasteurella

Mnemonic: “CAN PP”

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4
Q

Positive result in Nitrate reduction test:

A

Red, or no color after addition of zinc dust

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5
Q

Positive result in Carbohydrate Fermentation test:

A

With phenol red indicator, change from RED to YELLOW.

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6
Q

A test for slow lactose fermentation (late). Helpful in differentiating Citrobacter (+) from most Salmonella (-)

A

ONPG test

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7
Q

Positive result in ONPG:

A

Yellow color

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8
Q

Enzymes present in rapid lactose fermenters:

A

B-galactosidase
Permease

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9
Q

Enzymes present in late lactose fermenters:

A

B-galactosidase only

Non-lactose fermenters does not possess B-galactosidase and Permease

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10
Q

Good test to differentiate Salmonella from Shigella:

A

H2S production test

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11
Q

H2S production test positive result:

A

Black precipitate

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12
Q

Ratio of sugars present in Triple Sugar Iron (TSI) Agar:”

A

Glu : Lac: Suc
1:10:10

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13
Q

Growth in the slant is _____ (Aerobic/Anaerobic)

A

Slant = Aerobic

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14
Q

Growth in the Butt is _____ (Aerobic/Anaerobic)

A

Butt = Anaerobic

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15
Q

In Butt/Slant (TSI), fermentation happens in:

A

Butt

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16
Q

In TSI Agar

Yellow =
Pink =

Pink Butt =
Yellow Butt =

A

Yellow = Acid (A)
Pink = Alkaline (K)

Pink Butt = glucose not fermented
Yellow Butt = glucose fermented

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17
Q

In TSI

If only glucose were fermented, the TSI result is:

A

Glucose only = K/A (Pink/Yellow) or (Alkaline/Acid)

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18
Q

In TSI

If glucose and another sugar (lactose/sucrose) were fermented, the TSI result is:

A

Glucose + Lactose/Sucrose = A/A (Yellow/Yellow) or (Acid/Acid)

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19
Q

In TSI

Non-fermenters TSI result would be:

A

Non-fermenters = K/K (Pink/Pink) or (Alkaline/Alkaline)

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20
Q

Difference between Proteus vulgaris and Proteus mirabilis:

A

Proteus vulgaris = Sucrose fermenter (A/A)
Proteus mirabilis = Non-sucrose fermenter (K/A)

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21
Q

Citrobacter TSI:

A

K/A or A/A

Citrobacter is a Late-Lactose-Fermenter. Initially, it’s TSI is K/A . In prolonged incubation, it’s TSI will turn into A/A

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22
Q

Salmonella and Shigella TSI:

A

Salmonella & Shigella = K/A

Salmonella = K/A with black precipitate (H2S +)
Shigella = K/A; no black precipitate

one way to differentiate Salmonella and Shigella is the H2S production test;
Salmonella (H2S+)
Shigella (H2S-)

23
Q

Indole positive organisms:

A
  • Escherichia coli
  • P. vulgaris
  • Klebsiella oxytoca

Klebsiella oxytoca is the ONLY Indole(+) Klebsiella
another way to differentiate P. vulgaris and P. mirabilis: P. vulgaris is indole (+) while P. mirabilis is indole (-)

P. vulgaris = A/A; indole (+)
P. mirabilis = K/A; indole (-)

24
Q

Enzyme that is produced in indole test:

A

Tryptophanase

25
Q

Test to diffrentiate lactose-fermenting Enterobacteriaceae:

A

Methyl-Red - Vogues Proskauer

26
Q

Test for the identification of lactose-fermenting Enterobacteriaceae:

A

Citrate

27
Q

Positive result in Citrate test:

A

Growth with an intense BLUE COLOR on the slant.

28
Q

E. coli IMVIC reaction:

A

E. coli = (+) (+) (-) (-)

29
Q

K. pneumoniae IMVIC reaction:

A

K. pneumoniae = (-) (-) (+) (+)

30
Q

E. aerogenes & E. cloacae IMVIC reaction:

A

E. aerogenes & E. cloacae = (-) (-) (+) (+)

30
Q

K. oxytoca IMVIC reaction:

A

K. oxytoca = (+) (-) (+) (+)

30
Q

K. ozanae IMVIC reaction:

A

K. ozanae = (-) (+) (-) (-)

31
Q

E. agglomerans IMVIC reaction:

A

E. agglomerans = (-) (-) (-) (-)

32
Q

Rapid Urease Producers:

A

Proteus
Morganella

33
Q

Positive result in Urease test:

A

Yellow to PINK

34
Q

Urease-positive organisms:

A
  • Proteus
  • Providencia rettgeri
  • Morganella
  • Cryptococcus (Fungi)
  • H. pylori
  • Ureaplasma
  • Nocardia
  • Klebsiella
  • S. epidermidis
  • S. saprophyticus

mnemonic: “PP Mo CHUNKSS”

35
Q

Phenylalanine deaminase (PD) positive result:

A

GREEN COLOR after the addition of ferric chloride

36
Q

PD-positive organisms:

A
  • Proteus
  • Providencia
  • Morganella
  • Klebsiella (SLOW)

mnemonic: “PD PP Mo, K?” / “PD PP Mo GREEN, K?”

37
Q

Decarboxylase reaction positive result:

A

yellow to PURPLE

38
Q

In Decarboxylase reactions

Klebsiella =
Enterobacter =

A

Klebsiella = LDC (+) (Lysine Decarboxylase)
Enterobacter = ODC (+) (Ornithine Decarboxylase)

39
Q

Motility of bacteria is determined using a ______ media

A

motility: SEMISOLID media

40
Q

Non-motile Enterobacteriaceae:

A
  • Shigella
  • Klebsiella
  • Yersinia enterocolitica (Non-motile @37C) (Motile @ 22C)

Mnemonic: “SKY”

41
Q

This test is used to determine the ability of an organism to use acetamide as the sole source of carbon

A

Acetamide Utilization Test

42
Q

Organism positive for Acetamide utilization test:

A

Pseudomonas aeruginosa (+)

43
Q

Positive result of acetamide utilization test:

A

BLUE COLOR

44
Q

This test is used to determine if an organism can use acetate as the sole source of carbon:

A

Acetate utilization test

45
Q

Positive result of acetate utilization test:

A

BLUE COLOR

46
Q

Organism positive for acetate utilization test:

A

E. coli (+)

47
Q

H2S positive organisms:

A
  • Proteus
  • Edwardsiella
  • Citrobacter
  • Salmonella

Mnemonic: “PECS”

48
Q

VP positive organisms:

A
  • Klebsiella
  • Enterobacter
  • Serratia
  • Hafnia

Mnemonic: “KESH”

49
Q

Lactose-Fermenter organisms:

A
  • Escherichia
  • Klebsiella
  • Enterobacter

Mnemonic: “EKE”

50
Q

Late-Lactose-Fermenter organisms:

A
  • Citrobacter
  • Salmonella arizona
  • Shigella sonnei
  • Serratia
  • Hafnia
  • Yersinia

Mnemonic: “CSSSHY”

51
Q

Non-Lactose Fermenter organisms:

A
  • Proteus
  • Providencia
  • Morganella
  • Salmonella
  • Erwinia
  • Shigella
  • Edwardsiella

Mnemonic: “PP Mo SESE”

CLINICAL MICROBIOLOGY - BACTERIOLOGY (TopnotcherQT) (27 decks)

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