SPECIAL BACTERIOLOGIC MEDIA

Question 1

Bacteriologic media for Corynebacterium diphtheriae

Answer 1

Cystine-tellurite blood agar
Loeffler medium
Tindale agar

Question 2

Selective medium for Yersinia enterocolitica, Aeromonas and Plesiomonas shigelloides

Answer 2

Cefsulodin-irgasan novobiocin agar (CIN)

Yer”CIN”ia

Question 2

Enrichment medium for recovery and identification of C. diphtheria; promotes the development of characteristic granules of C. diphtheria

Answer 2

Loeffler medium

Question 3

In CIN, Y. enterocolitica ferments _____ and appears as red _____ colonies surrounded by colorless halo.

Answer 3

ferments mannitol;
appears as red “Bull’s eye” colonies surrounded by colorless halo

Question 4

Enrichment medium for recovery of Vibrio and Aeromonas from stool

Answer 4

Alkaline peptone water

Question 5

Selective differential for vibrios

Answer 5

Thiosulfate citrate bile salts sucrose agar (TCBS)

Question 6

V. cholerae in TCBS ferments ______ and produces ______ colonies

Answer 6

V. cholerae in TCBS ferments SUCROSE and produces YELLOW colonies

Question 7

Used to speciate Haemophilus

Answer 7

Rabbit blood agar

H. hemolyticus and H. parahemolyticus are hemolytic

Question 8

Selective enrichment medium for isolation of Bordetella pertussis

Answer 8

Bordet-Gengou

Question 9

Specimen of choice for isolation of Bordetella pertusis:

Answer 9

Nasopharyngeal swab

Question 10

Bordetella colonies resemble ______.

Answer 10

mercury droplets

Question 11

Enrichment medium for isolation of Legionella

Answer 11

Buffered charcoal yeast extract agar (BCYE)

Question 12

Mediums used for isolation of Gardnerella vaginalis
(G. vaginalis)

Answer 12

1. Vaginalis agar (V agar)
2. Human Tween agar (HBT)

Question 13

Nonselective enrichment medium for isolation of G. vaginalis:

Answer 13

Vaginalis agar (V agar)

Question 14

G. vaginalis in V agar and HBT produces ________.

Answer 14

diffuse beta hemolysis

Question 15

Selective enrichment mediums used to culture mycobacteria:

Answer 15

1. Lowenstein-Jensen
2. Middlebrook 7H10 and 7H11

Question 16

Fletcher medium contains:

Answer 16

Rabbit serum

Question 17

Enrichment medium for isolation of Leptospira from blood, CSF, and urine

Answer 17

Fletcher medium