Rabies Flashcards
What family of virus is rabies
Rhabdoviridae
- What order of virus is Rabies?
a. Mononegavirales
- How far back is rabies known to have existed?
a. 2,300 BC – Babylon
- What shape is the rabies virus?
a. bullet
- How many kilobases id the RNA genome of the Lyssaviruses?
a. 12 kb
- How many proteins does the Lyssaviruses encode?
a. 5
- What are the five viral proteins that the Lyssaviruses encodes?
a. nucleoprotein N,
b. phosphoprotein P,
c. matrix protein M,
d. glycoprotein G
e. polymerase L
- What length is the Lyssaviruses?
a. 100-300 nm
- What is the width of the Lyssaviruses?
a. 75nm
- How many struntural and functional units does the Lyssaviruses have? and what are they?
a. two
b. lipid bilayer
c. ribonucleokapsid (RNP)
- Is the Lyssaviruses enveloped or unenveloped?
a. Enveloped
- What is the envelope of the Lyssaviruses made of?
a. The lipid bilayer from the host cell builds the outer envelope.
b. It is covered with spike like projections corresponding to G-Protein trimers, which recognise and bind cell receptors.
c. The G-Protein is essential for lyssavirus pathogenicity and for the induction of the immune response.
- What is essential for lyssavirus pathogenicity and for the induction of the immune response?
a. G-proteins
What is the The internal ribonucleokapsid (RNP) made up of?
a. helical structure and is composed of the genomic RNA intimately associated with protein N, polymerase L and its cofactor protein P (formerly named M1).
b. The ribonucleocapsid complex ensures genome transcription and replication in the cytoplasm.
c. The matrix protein M (formerly named M2) occupies an intermediate position between the ribonucleocapsid and the envelope, and is responsible for virus budding and the bullet-shaped morphology.
- The _______ _________ ensures genome transcription and replication in the cytoplasm.
a. ribonucleocapsid complex
- _______ BC – Babylon - dog owners fined for human deaths caused by their dogs.
a. 2,300BC
- _________ BC - Homer likens Hector to a “raging dog” in The Iliad
a. 800-700 BC
- ______- 1st large rabies outbreak reported (Germany)
1271
- ______- 1st rabies case reported in the Americas by a priest in Mexico
1703
- What are the major reseviours of rabies?
a. Canidae - dogs, foxes, raccoon dogs, jackals, coyotes & wolves
b. - Mustelidae – skunks
c. - Viverridae – mongooses
d. - Procyonidae - raccoons
e. - Chiroptera - Insectivorous, hematophagous & frugivorous bats
- What animals are thought to be rabies vectors?
a. Cats: bobocats and cougars
- What animals are considered high risk and are reseviour species?
a. • Dog (wild or domestic – fox, coyote, wolf, etc.)Dog (wild or domestic – fox, coyote, wolf, etc.)
b. •• Raccoon, Skunk, MongooseRaccoon, Skunk, Mongoose
c. •• Cow (South America only)Cow (South America only)
d. •• Bat ( particularly vampire)
- What constitutes a reseviour species?
a. Virus passed amongst these animals maintaining it in the population for long periods of time
- How is populatin density determined for zoonotic disease?
a. Number of animals per square foot of ground
- Higher population density -_________ rate of transmission
increased
- How does herd immunity help affect zoonotic disease?
a. Immune animals protect susceptible individuals within their herd
- What animal is 90% of rabies exposure from?
a. DOGGOS
- ________% of human rabies deaths result from dog bite
99%
- How many deaths from rabies are there on average per year globally?
a. >55,000
- What is the prevalence of rabies per 100,000 in the following countries?
a. Africa (rural): _____/100,000
b. India (rural): _____/100,000
c. Pakistan: _____/100,000
d. China: _______/100,000
a. Africa (rural): 3.6/100,000
b. India (rural): 2.5/100,000
c. Pakistan: 1.2/100,000
d. China: 0.2/100,000
- How many cases per year in the phillipines is there of rabies?
a. 350/450 cases/yr
- How many dog bite incidents is there in the phillipines each year?
a. 140,000 – 560,000/yr
- 53% cases – _____ to ______ yr age group in the phillipines
5-14
- What is the percentage breakdown of the animals infected in the phillipines?
a. Dogs 98%
i. - pet 88%
ii. - stray 10%
b. - cats 2%
- What is the exposure rate to rabies in the United states each year?
a. 20,000- 40,000
- What are the average number of human rabies cases each year in the united states?
a. 1-8 cases
- How many cases of animal rabies cases are found each year?
a. 7,000 – 10,000
- ________ rabies has been eliminated in the USA.
a. Dog
- Which state in the USA does not have rabies?
a. Hawwaii
- What animals have rabies in the USA?
a. Raccooons
b. Skunks
c. Foxes
d. Mongoose
e. Bats
- Can there be asymptomatic carries of rabies?
a. Yes only in animals known
- How long can rabies remain infectious after an aminal dies?
a. <24 at 20ᵒ
- How is rabies transmitted(4)?
a. Wet slavia
b. Nervous tissue
c. Aerosol in bat caves
d. Transplants
- Is dry saliva of rabies infectcious?
a. NOPE
- In animals rabies can be transmitted rabies can be transmitted ___________
a. transplacentally
- Can human to human transmission of rabies occur?
a. Not to date
- Rabies has never been isolated form what tissue type?
a. Blood
- What are the chances (%) of developing rabies after a bite?
a. 5-80%
- What are the chances of developing rabies after a bite dependant on?
a. Severity of exposure
b. • Location of the bite
c. • The biting animal
- Upon entering the body where does the rabies virus first remain dormant and replicate?
a. The muscle at the site of the bite
- Once the virus has replicated in the muscle where does the virus go?
a. Nerves in the peripheral NS
- How does the rabies virus move through the nerves in PNS?
a. Retrograde transport
- What is retrograde transport?
a. Retrograde axonal transport conveys materials from axon to cell body.
- Once in the PNS the rabies virus moves to the ________ and _______?
a. Dorsal root ganglion
b. replicates
- Upon completion fo replication in the dorsal root ganglion where does the virus move to?
a. The brain
- Once the brain is infected the virus will migrate to the nerves of other tissues including (3)?
a. Eye
b. Kidney
c. Salivary glands
- What is the incubation period of rabies?
a. 5 days up to 2 year
- What is the time frame for neutralising a rabies wound before it is too late to treat?
a. Max 14 days
- What are the symptoms with furious (Encephalitic form) rabies?
a. Symptoms - agitation, hydrophobia, extreme irritability, hyper-excitability periods fluctuate with lucidity
b. Vital signs abnormal – tachycardia, tachypnea, fever
c. Aerophobia
d. Hallucinations
e. Seziures
f. Ataxia
g. Foacl weakness
- What form of rabies is furious rabies?
a. Encephalitic form
- What is aerobphobia?
a. extreme fear of air in motion.
b. Can result in violent muscle spasms in neck & pharynx
- How is furious rabies (Encephalitic form) Treated?
a. NONE once it goes into the nervous system
- Once symptoms of furious rabies (Encephalitic form) occur what can yeeee FECKIN die?
a. 3-10 days
- If given ICU treatment and you have furious rabies (Encephalitic form) ho long can you survive before dying?
a. 4 months
- How many people have servived furious rabies (Encephalitic form)? And why did they survive?
a. 6
b. Had pre or post-exposure prophylaxis before onset of symptoms
- What are the two types of rabies?
a. furious (Encephalitic form)
b. Dumb Rabies (Rage tranquille)
- What type of rabies is dumb rabies?
a. (Rage tranquille)
- Which form of rabies is difficult diagnose?
a. (Rage tranquille)
b. DUMB
- What is Dumb Rabies (Rage tranquille) also sometime referred to as?
a. Paralytic rabies
- What other illness is Dumb Rabies (Rage tranquille) confused with?
a. Guillain-Barre syndrome
- What is the most common farm animal infected with rabies. And what other animals can be infected?
a. Cows
b. horses, sheep, pigs & goats
- What symptoms do cows develop when they are infected with rabies?
a. develop hoarse bellow, drool & swallow abnormally
b. - Some only show depression, weakness or partial paralysis of hindquarters
c. - Hours or days later- animal goes down, develop convulsive seizures & die
- What symptoms do sheep develop when they are infected with rabies?
a. - Some only show depression, weakness or partial paralysis of hindquarters
b. - Hours or days later- animal goes down, develop convulsive seizures & die
c. Head pressed against something
d. No vocalisation
- What symptoms do horses develop when they are infected with rabies?
a. Rare in horses - exposed horses very sensitive & susceptible
b. Clinical signs – paralytic form
c. Off feed, exhibit depression, excessive salivation, difficulty swallowing, lack of coordination, aggressive behaviour, hyper-excitability, colic, convulsions or paralysis
- Is there a vaccine for rabies? Is there a treatment for rabies?
a. Vaccine yes
b. Tratment no
- How is rabies diagnosed?
a. Brain biopsy - Negri Bodies found
b. Saliva test - inaccurate
c. History of animal exposure & typical neurologic clinical signs
d. IFAT - Laboratory diagnosis Gold standard
e. Humans - antemortem detection of virus/viral amplicons, antibodies or antigens (sera, CSF, saliva, nuchal biopsy)
f. Rapid immunodiagnostic test (RIDT)
g. RTPCR
- How is rabies detected in brain biopsy?
a. Negri Bodies found
- What test is inaccurate for detecting rabies?
a. Salvia test
- What is the Lab gold standard rabies test?
a. IFAT
- What is the Lab gold standard rabies test?
a. IFAT
- Which diagnostic test is typically used for completing surveillance for rabeies?
a. Rapid immunodiagnostic test (RIDT)
- If a Rapid immunodiagnostic test (RIDT) is positive what colour is it and it is negative what colour is it?
a. Neg purple
b. Positive pink
- What prevalence of rabies is there in the UK and Ireland?
a. European bat lyssavirus (EBLV) 2 - detected at low prevalence in Daubenton’s bats