anthrax Flashcards
1
Q
- What are the two forms that anthrax exists in?
A
a. Vegetative
b. Spores
2
Q
- Anthrax come from with bacterium?
A
a. .Bacillus anthracis
3
Q
- Where in the world can you find Bacillus anthracis?
A
a. Globally distributed
4
Q
- How many strains of Bacillus anthracis are there?
A
a. 1,200
5
Q
- What is the incubation period of Bacillus anthracis?
A
a. 3-7 days
6
Q
- The Bacillus anthracis bacterium causes severe disease in what animals?
A
a. Cattle
b. Sheep
c. Goats
d. Horses
7
Q
- The Bacillus anthracis bacterium causes moderate disease in the what animals?
A
a. Pigs
b. Dogs
c. Cats
8
Q
- Does Bacillus anthracis affect birds?
A
a. Yes. 7 species
9
Q
- In what form is Bacillus anthracis found in the environment?
A
a. Spore form
10
Q
- How long can Bacillus anthracis survive in the soil?
A
a. Decades
11
Q
- The Bacillus anthracis is highly _________ to biological extremes.
A
a. Resisitant
12
Q
Bacillus anthracis is an ________ parasite
A
obligate
13
Q
- Where do the Bacillus anthracis spores germanate?
A
a. The host
14
Q
- What is the lethal inhilation of Bacillus anthracis spores by humans?
A
a. 2,500 - 55,000
15
Q
- What does the Bacillus anthracis spores requires to form?
A
a. Oxygen
b. Nutrient poor environment
16
Q
- What is the endospore cycle?
A
a. Inhalation (abrasion and ingestion)
b. Macrophage: phagocytose
c. Regional lymph nodes
d. Germinate inside the macrophage
e. Vegetative forms
f. Release
g. Multiply in the lymphatics
h. Blood stream
i. 107 – 108
j. Septicaemia
k. Death
l. Inhalation
17
Q
- What does the Bacillus anthracis have on the exterior of its cells, and what does it protect it from?
A
a. A coating
b. Antibodies
18
Q
What enviroments conditions can lead to an anthrax break out and why?
A
Heavy Rainfall followed by a dry period, this is because spores washed out of soil, and spores are then concentrated in a particular area
18
Q
What enviroments conditions can lead to an anthrax break out and why?
A
Heavy Rainfall followed by a dry period, this is because spores washed out of soil, and spores are then concentrated in a particular area
19
Q
- What do Bacillus anthracis release as they multiple in the macrophages?
A
a. Toxins
20
Q
- What causes the death from Bacillus anthracis?
A
a. Toxin build up
21
Q
How long has anthrax bee shown to survive?
A
50 years
22
Q
- When do Bacillus anthracis go into their dormant vegetative state?
A
a. When the animal dies and they have decomposed
23
Q
- How may protein coats does Bacillus anthracis spores have?
A
a. Three
24
21. What is the Bacillus anthracis DNA protected by?
a. It is contained within a core
25
22. What is Edema factor (EF)?
a. Is an 89-kDa protein secreted by Bacillus anthracis, the Gram-positive bacterium that cause anthrax. EF belongs to a family of bacterial toxins that can specifically elevate the intracellular cyclic AMP (cAMP) level, which is the prototypic second messenger that regulates diverse cellular responses.
26
23. What makes up the Edema toxin?
a. Edema Factor + Protective Ag
27
24. Increased cellular ________ --> Edema --> Impaired ________ function
a. cAMP
| b. Neutophil
28
24. Increased cellular ________ --> Edema --> Impaired ________ function
a. cAMP
| b. Neutophil
29
25. What happens when Calmodulin dependent adenyl cyclase is activated?
a. it catalyses the conversion of ATP to cyclic AMP, which leads to an increase in intracellular levels of cyclic AMP.
30
26. When Calmodulin dependent adenyl cyclase is activated, it depletes ATP from ___________.
a. Macrophages
31
what is gerH locus?
tricitronic operon in B. anthracis help germination with help from co-germinates unwittingly provided by the macrophages
32
27. What is the lethal toxin made from?
a. Lethal factor + Protective Ag
33
28. What does the lethal factor do?
a. Zinc metallo proteases that inactivates protein kinases
| b. Stimulates Macrophages – TNF alpha & IL – 1 beta – Shock & Death
34
29. What is the result of death from anthrax toxin?
a. Death due to O2 depletion, secondary shock, increased vascular permeability, respiratory failure & cardiac failure
35
30. What are Mitogen-activated protein kinase?
a. Mitogen-activated protein kinases (MAPKs) are serine and threonine protein kinases that are highly conserved in eukaryotes, and are involved in signal transduction pathways that modulate physiological and pathophysiological cell responses
36
31. In Sverdlovsk, ______, 1979, 94 individuals were taken ill and _____ died.
a. Russia
| b. 64 died
37
What does the cCAMP increase result in,
prevents neutrifil, reduces AMP, and prevents the water influx homeosttis leading to adema
38
32. In South Africa, between 1978-80, 10,738 human cases with _______ known deaths, _______ of cattle dies, this took place on the Black _____ lands only.
a. 182
b. Thousands
c. Tribal
39
33. In _____ 1993- ______ Shinrikyo released anthrax.
a. Tokyo
| b. Aum
40
34. In USA _______ - anthrax contaminated letters mailed to prominent US figures
a. 20001
41
35. What were the cases that arose from the 2001 Anthrax attack: ________ cutaneous ______ inhalation and _________ deaths?
a. 11 cutaneous
b. 11 inhalations
c. 5 deaths
42
36. How old was the infant that receive a cutaneous lesion from the 2001 anthrax attack?
a. 7 month old boy
43
37. Where is anthrax most common?
a. Africa: Zimbabwe
b. South east asia
c. China
d. South America
e. Turkey
f. Pakastan
g. India
44
38. How do grazing animal become infected with anthrax?
a. Eating spores in the soil
45
39. Can be spread from an infected animal to a _______
a. Heard
46
40. How is anthrax spread?
a. Biting flies
b. Vulture
c. Contaminated water
47
41. The spread from live stock to farm workers is ________.
a. Rare
48
42. Laboratory workers getting infected from working with spores is _______.
a. Rare
49
43. People being infected through contaminated animal products is _______.
a. Rare
50
44. Processors of wool, hair, hides, bones, or other animal products is now ______.
a. Rare
51
45. What are the three routes of transmission?
a. Inhalation
b. Direct contact with soil or animal product
c. Gastrointestinal
52
46. How can animals transmit the disease?
a. Haemorrhagic exudate from mouth nose or anus
53
47. Sporulation doesn’t occur in closed _______ Spores viable for ________.
a. Carcass
| b. Decades
54
48. Herbivores primary source of contamination comes from _____________. Carnivores major source of contamination comes from ___________. They can both contract through _________ and infection through _________.
a. Dirt
b. Contaminated meat
c. Inhalation
d. Insect bite
55
49. What species are at greatest risk of infection?
a. Ruminants
56
50. There are three forms of clinal manifestation _________, ________ and __________ chronic.
a. Peracute
b. Acute
c. Subacute chronic
57
51. What animals are most likely to get a peracute infection?
a. Ruminants: cattle sheep goats and antelope
58
52. What animals are most affected by the acute infection?
a. Ruminants and equine
59
53. What animals are most affected by the subacute-chronic infections?
a. Swine
b. Dogs
c. Cats
60
54. __________ anthrax infection accounts for 95% of the global cases.
a. Cutaneous
61
55. What is the incubatory period of cutaneous anthrax?
a. 1-12 days
62
56. How do you get infected from cutaneous anthrax?
a. Through an abrasion
63
57. What are the four stages of infection from cutaneous anthrax?
a. Papule vesicle ulcer eschar
64
58. What are the clinical manifestations of cutaneous anthrax?
a. Oedema
b. Redness
c. Necrosis
d. Ulceration
65
59. What percentage of those infected with cutaneous anthrax die with antibiotics and without?
a. Without: 20%
| b. With 1%
66
60. How is cutaneous anthrax diagnosed?
a. Gram stain
b. PCR
c. Culture of vesicular fluids
d. Exudate
e. Eschar
f. Blood culture
g. Biopsy
67
61. What is the incubation period of inhalation anthrax?
a. 1-7 days
68
What does contiguous mean?
when herds meet other heards
69
62. What percentage of individuals with inhalation anthrax also get?
a. 50% meningitis
70
63. What is a prodrome?
a. Prodrome: a brief sensation of déjà vu before the episode indicates a focal onset seizure; a feeling of lightheadness, sweatiness and visual fading suggests syncope.
71
64. What type of anthrax do you get a brief prodome?
a. Inhalation anthrax
72
65. What symptoms do you get with pharyngeal anthrax?
a. Fever
b. Pharyngitis
c. Neck swelling
d. Haemorrhagic mediastinitis
73
66. What is the fatality of inhalation anthrax with antibiotics/without?
a. Without 97%
| b. With 75%
74
67. How is inhalation anthrax diagnosed?
a. Chest X-ray
i. idened mediastinum, pleural
ii. effusions, infiltrates, pulmonary congestion
b. Tissue biopdy for ICC
c. Gram Stain
d. PCR
e. Culture
f. Pleural fluid cell block
75
68. What type of agar can Bacillus anthracis grow?
a. Blood agar
76
69. What type of colonies does Bacillus anthracis grow?
a. non-haemolytic gray-white colonies
77
70. What can Fluorescent antibody stain be used for?
a. Identification
78
71. What clinical symptoms develop from Gastrointestinal Anthrax?
a. bloody vomit or diarrhoea, followed by fever & signs of septicaemia
b. Sometimes seen as oropharyngeal ulcerations with cervical adenopathy & fever
79
72. What is the incubation period of Gastrointestinal Anthrax?
a. 1-7 days
80
73. What percentage of Gastrointestinal Anthrax cases are fatal?
a. 25-80%
81
74. How is Gastrointestinal Anthrax disgnosed?
a. Blood cultures
| b. Oropharyngeal (OP) swab collection
82
75. What differential diagnosis is there fore cutaneous anthrax?
a. Boils, Erysipelas, Cutaneous TB, Leprosy, Plague, Vaccinia, Rickettsial pox, tularemia
83
76. What differential diagnosis is there fore intestinal anthrax?
a. Typhoid fever, Acute Gastroenteritis, Tularemia, Peritonitis, Peptic ulcer, Mechanical obstruction
84
77. What differential diagnosis is there for pulmonary anthrax?
a. iral pneumonia, Mycoplasma. Psittacosis, Legionnaires disease, Q fever, Histoplasmosis, Coccidiodomycosis, Silicosis, Sarcoidosis
85
78. What treatment is there for anthrax and resistance associated (3)?
a. Penicillin: most strains suceptible
b. Ciproflocin: no resistance as of 2001
c. Doxycycline:
86
79. What is the treatment length?
a. 6- days
87
80. What was can infection be controlled and prevented?
a. Humans protected by preventing disease in animals
i. −- Veterinary supervision
ii. −- Trade restrictions
b. •Improved industry standards
c. •Safety practices in laboratories
d. •Post-exposure antibiotic prophylaxis
e. •Vaccination of high risk groups
f. •Anthrax Vaccine Adsorbed (AVA)
88
81. What decontamination methods are there for anthrax?
a. Moist heat kills – Vegetative cells 60 0 C X 30 minutes
i. Spores 100 0 C X 10 minutes
b. 4% Formaldehyde kills spores
c. 4% KMnO4 kills spores
d. Hypochlorite ( 0.5%) commercially available kills spores
89
82. What is Linkage disequilibrium?
a. In population genetics, linkage disequilibrium (LD) is the non-random association of alleles at different loci in a given population. Loci are said to be in linkage disequilibrium when the frequency of association of their different alleles is higher or lower than what would be expected if the loci were independent and associated randomly.
90
83. What would happen if 50kg of spores where released into an urban area of 5 million people?
a. 250,000 cases of anthrax
| b. 100,000 deaths
91
84. If 100 kg of spores were released upwind of Washington D.C how many people would die?
a. 130,000 to 3 million
92
What is a warning sign that an animal has anthrax (peracute)?
stumbling and trembling, falling over
