Pyruvate Dehydrogenase Flashcards
What’s important (overview) about the Pyruvate Dehydrogenase complex?
There are two main fates of Pyruvate, Fermentation or Citric Acid Cycle. Where do these reactions take place respectively?
Where is ATP generation in a mitochondrion? Where is Pyruvate oxidation and CAC done in the mitochondrion?
What two common cells don’t have mitochondria? Why?
What does Pyruvate Dehydrogenase do chemically?
1st main carbon that’s being decarboxylated.
Highly regulated!
What are the 3 Enzymes in the Pyruvate Dehydrogenase reaction?
What are the 5 cofactors in the Pyruvate Dehydrogenase reaction? Where are they located and what do they do?
Overview: what are the 5 reactions of the Pyruvate dehydrogenase complex.
What is the net outcome of the Pyruvate dehydrogenase complex?
Explain the 1st reaction of the Pyruvate Dehydrogenase complex.
What enzyme/cofactors are apart of it.
How does it work?
Know the cofactor well!!!
Explain the 2nd reaction of the Pyruvate Dehydrogenase complex.
What enzyme/cofactors are apart of it.
How does it work?
How does E1 and E2 interact in the Pyruvate dehydrogenase complex? (Between step 1 and 2)
Explain the 3rd reaction of the Pyruvate Dehydrogenase complex.
What enzyme/cofactors are apart of it.
How does it work?
Explain the 4th/5th reaction of the Pyruvate Dehydrogenase complex.
What enzyme/cofactors are apart of it.
How does it work?
How are they different?
Redox are different!
NAD+: accepts 2 e- at the same time in a single transfer.
FAD: Put on 2 e- one after the other! Not together.
Define substrate channeling.
What are the two ways PDH (Pyruvate Dehydrogenase complex) are regulated. Which form is active/inactive?
Summary slide:
