Enzyme Kinetics I: Michaelis-Menton Flashcards
What are some fundamental scientific reasons kinetics are important? (6)
What are some practical reasons for why kinetics are important? (5)
What does “k” represent in this equation? And what does it tell us?
The rate that A is being converted to B.
What does this sign stand for?
Standard free energy of activation.
What are the two ways to speed up a reaction?
- Increase temperature (T)
- Decrease standard free energy of activation (Delta G not, double dagger.)
Both increase k = reaction rate.
How do enzymes increase reaction rates?
Bonus: do enzymes increase rates only one way, or both?
They DO NOT change equilibrium concentrations nor energy requirements for products/reactants. Only the transition state energy required.
What are these two graphs showing?
What is V max?
What is Km?
V Max = Horizontal asymptote that this reaction velocity approaches; ** maximum velocity of reaction that we reach given an infinite levels of substrate concentration**
Km= the amount of substrate that gives us half of this reaction velocity.
Fill in the blanks, make sure you understand why things go where they do.
What is the initial velocity equation? (Probably better to memorize but here’s hint.) the Michaelis-Menten Equation.
Optional
What are the two unknowns in the Michaelis-Menten Equation.
What is the Michaelis-Menten Equation? What are the two important consequences from the equation?
Fill in blanks
What is the point of the Lineweaver-Burk plot?
If it isn’t a straight line, you know it’s not an equation that follows Michaelis-Mentens equation.
What are these graphs showing us?
Left: the basic equation of enzyme kinetics rates.
Right= plot that gives us the KM and Vmax of the left graft.
