Mitochondrial Electron Transport Flashcards
What’s happening here?
Overview: how many complexes make up the ETC? What’s the net reaction assuming NADH is the reactant?
All are integral proteins!
The electron transport chain is a long series of ______ reactions.
Always moving down hill! Slowly!
What does: E° ; stand for? (It’s purpose not how it’s measured)
What is: E° ; measured? (It’s measure not what it stands for)
What’s the most hungry for electrons? What’s the least?
Most: 1/2O2+2H+2e -> H2O
Least: H+e -> 1/2H2
What is Faraday’s constant.
96,485 J/mol*V
How do you find Delta G°’ using E°?
What are the groupings?
What do orange arrows show?
What is the y axis showing?
Complexes.
Where protons are transferred.
Standard reduction potential.
How many subunits are in each complex?
What are the soluble e- carriers in the ETC? Hint: 2
What are the 4 different types of electron carriers in the ETC?
All about Complex 1:
What does it use for energy? (What accepts the electrons in the complex?)
What does it use the energy to do?
What side is the energy transfer on?
How many subunits are there?
What’s getting oxidized? What about reduced? (What’s the overall reaction?)
NADH getting oxidaized.
Q getting reduced.
What do Flavin Mononucleotide FMN and FAD have in common regarding Complex 1, what is its role and how does it work?
How is it working in complex 2?
Note: it is not something we can make ourselves. (B2!!!), must acquire through eating plants.
Iron sulfur (FeS) clusters do what in the ETC?
Iron is the redox active part.
Remember cysteines usually coordinate it!
Explain how Coenzyme Q works in complex 1.
Give the overall reaction of complex 1.
Define what complex 2 is and how it works, and why it differs from complex 1.
Succionaste dehydrogenase! (Look at previous flash cards to know how that works).
Complex 3: what proteins do they have that interact with heme groups? What do they do with them?
What is the Q cycle?
Between complex 3 and 4, what protein does the pathway use? What does it do? What side is this protein on?
Explain how Complex 4 works.
What’s the overall reaction?
(Pathway isn’t yet fully understood lol a lot of this lecture ;(, just memorize what’s in the slide!)
How do we analyze the intermediates of this process? (The one way we learned about)
Antimycin A inhibits what complex where?
Rotenone inhibits what, where?
Analyzing these inhibitors allow us to understand what?
Summary slide:
