Pseudomonas Flashcards
Pseudomonas: Gram ________? Shape?
Gram Negative, Rods
Pseudomonas: Aerobic/Non-aerobic?
Obligate aerobe
Pseudomonas: Motile/Non-motile?
Motile
Pseudomonas: Distribution? Opportunist?
Ubiquitous - Worldwide - Remarkable capacity to adapt & thrive in diverse ecological niches **Opportunistic Pathogen**
Pseudomonas: What is the strain of veterinary importance?
P. aeruginosa - Secondary agent
Pseudomonas: Does it exhibit antimicrobial sensitivity?
**Significant antimicrobial resistance** R plasmids!
Pseudomonas: Places it can be found in the environment?
Wet, poorly aerated environments -Surgery areas w/support bags -Hoses in anesthetic machine -Mastitis preparations/infusions -Semen extenders -Irrigation Fluids -Hydrotherapy baths -***Endotracheal tubes
Pseudomonas: Virulence Factors
-Adhesins (different types) -Capsule -Cell Wall LPS -Extracellular Slime/Biofilm -Siderophores (types) -Exotoxins -Others: Elastase, Proteases, Phospholipase C (hemolysin)
Pseudomonas: Types of Adhesins
-Fimbrial, Type IV pili, bind glycoproteins on epithelial cells -Non-Fimbrial (OMPs) -Affinity for mucin -Flagella & LPS - Affinity for Cl- channels
Pseudomonas: Types of Siderophores
Pyochelin & Pyoverdin
Pseudomonas: Types of Exotoxins
T3SS - **Exotoxin A** -Exotoxin S, T, U & Y
Pseudomonas: Exotoxin A function
Inhibits protein synthesis
Pseudomonas: Exotoxin S & T function
Affect actin cytoskeleton, interfere with phagocytosis
Pseudomonas: Exotoxin U function
Cytotoxic effect
Pseudomonas: Exotoxin Y function
Increases intracellular cAMP to cytopathic levels
Pseudomonas: Miscellaneous products
Bacteriocins & Pigments -Toxic to bacteria and eukaryotic cells
Pseudomonas: Bacteriocins (Pyocins)
Useful epidemiologically
Pseudomonas: Pigments (Pyocyanins)
Use Diagnostically -Produce O2 radicals toxic to eukaryotes & prokaryotes -Self-protected by catalase & superoxide dismutase