Pasteurella & Mannheimia

Question 1

Family Pasteurellaceae - description

Answer 1

Gram negative, coccobacilli, bipolarity, facultative anaerobes, oxidase positive (difference with Enterobacteriaceae), non-motile - catalase positive - reduce nitrates & ferment carbs

Question 2

Family Pasteurellaceae virulence factors - many

Answer 2

LPS - RTX toxin (glycine repeats & leukotoxin) -Rho activating toxin Pmt

Question 3

Which two species exhibit leukotoxin?

Answer 3

M. haemolytica & B trehalosi

Question 4

P. multicoda capsule toxin D

Answer 4

Atrophic rhinitis in swine, bordetella synergistic effect

Question 5

RTX toxin

Answer 5

Bovine leukocyttes & erythrocytes - hemolysis in vitro -***Bovine Shipping Fever!

Question 6

Pasteurella, Mannheimia - 3 general manifestations

Answer 6

Respiratory tract - septicemia - trauma associated

Question 7

Pasteurella, Mannhemia pathogenesis of pneumonia

Answer 7

Ruminants w/predisposing condition –> commensals of URT infect lung –> intensive inflammatory response (erythrocytes, neutrophils, mononuclear cells, fibrin deposition) –> Lkt, LPSm capsule, ion scavenging occurs

Question 8

Pasteurella, Mannheimia pathogenesis of atrophic rhinitis

Answer 8

Swine - If bordetella alone, will be self limiting infection. But: B. brochiseptica adheres–> damages epithelium (dermonecrotic toxin) –> P. multicoda toxins destroy nasal tissue –> Pmt –> destruction of bones & turbinates, chronic rhinitis (disturbed osteogenesis)

Question 9

Pasteurella, Mannheimia in Cattle

Answer 9

Shipping Fever - Hemorrhagic septicemia - Mastitis

Question 10

Shipping fever in cattle - causative species -

Answer 10

M. haemolytica, P. multicoda type A - B. trehalosi

Question 11

Shipping fever in cattle- concurrent infections

Answer 11

Infectious bovine rhinotracheitis virus -Mycoplasma - Ureaplasma - Bovine respiratory syncitial virus - Parainfluenza-3

Question 12

Pasteurella/ Mannheimia - Hemorrhagic septicemia in cattle - Causative species

Answer 12

P. multocida = REPORTABLE in USA

Question 13

Pasteurella/ Mannheimia - Mastitis in cattle - causative species

Answer 13

M. harmolytica, P. multocida

Question 14

Pasteurella/ Mannheimia - in Sheep & Goats

Answer 14

Septicemia - Enzootic pneumonia - Mastitis

Question 15

Pasteurella/ Mannheimia - Sheep/Goat septicemia - causative species

Answer 15

B. trehalosis, M. harmolytica - Lambs

Question 16

Pasteurella/ Mannheimia - Sheep/Goat enzootic pneumonia causative species

Answer 16

M. haemolytica - resembles bovine shipping fever

Question 17

Pasteurella/ Mannheimia - Sheep/Goat Mastitis causative species

Answer 17

M. haemolytica, B. trehalosis - “Blue Bag”

Question 18

Pasteurella/ Mannheimia - in Swine diseases

Answer 18

Atrophic rhinitis & pneumonia

Question 19

Pasteurella/ Mannheimia - Swine - Atrophic rhinitis

Answer 19

P. multocida & Bordetella bronchis, young pigs secondary pneumonia

Question 20

Pasteurella/ Mannheimia - Atropic rhinitis in Swine - virulence factors

Answer 20

Ammonia, Pmt, Dermonecrotic toxin

Question 21

Pasteurella/ Mannheimia - Pneumonia in swine

Answer 21

Fibrinous pneumonia - Often follows viral infections

Question 22

Pasteurella/ Mannheimia - In rabbits

Answer 22

Snuffles!!!! - Most important bacterial disease in rabbits - P. multocida type A, (Bordetella bronchiseptica is good DDx)

Question 23

Pasteurella/ Mannheimia - in birds

Answer 23

Fowl Cholera - P. multocida capsule Type A & F - REPORTABLE - Very rapidly fatal

Question 24

Pasteurella - Culture

Answer 24

VERY fastidious - Frows best on chocolate agar

Question 25

Mannheimia & bibersteinia- Culture

Answer 25

Grow on MacConkey, or Blood agar (bov/ov blood)

Question 26

Riemerella anatipestifier

Answer 26

“New Duck Disease”

Question 27

Photobacterium damsela

Answer 27

Septicemia disease in fish, dolphins, humans. Halophillic rod