(P2) Inf. disease: Actinomycosis (the oral cavity) (217)

Question 1

Name this slide:

1. What is that slotchy looking darker corner of specimen?
2. What are the small purple collections of cells

Answer 1

Actinomycosis (the oral cavity)

1. bacterial colony
2. neutrophils and macrophages

Question 2

Name the slide

3. What is the splotchy border of the figure called
4. What bacteria causes this?

Answer 2

Actinomycosis (the oral cavity)

3. amorphous border
4. Actinomyces group (A bovis)

gram-positive rods

Question 3

Actinomycosis (the oral cavity)

5. What distinct site does this infection occur in?
6. What other sites does this infection occur in?

Answer 3

5. Cervicofacial site (most common): dental work or jaw injury
6. oropharynx, GI tract, vagine

Question 4

Actinomycosis (the oral cavity)

How does the bacterium appear

macroscopically?

Answer 4

hard yellow grains known as sulfur granules

Question 5

Actinomycosis (the oral cavity)

1. What staining is shown on these slides?
2. What other staining can be used?

Answer 5

1. PAS
2. H&E

Question 6

Name this slide

What is the microbiological infectious classification of the organism that causes this infection?

Answer 6

Actinomycosis (the oral cavity)

gram positive rods

Question 7

What is the Bacteria of the oral cavity called

and

what disease does it cause?

Where does it reside?

Answer 7

Bacteria of actinomyces group (A bovis) gram-positive rods

Disease: Actinomycosis (the oral cavity)

The organisms reside as saprophytes in the human oropharynx, GIT and vagina

Question 8

Actinomycosis

1. This organism inhibits what parts of the body
2. This organism must be inoculated into what

Answer 8

1. These organisms inhibit the anaerobic surfaces of the mouth (tonsillar crypts, gingival and areas covered by dental plaque)
2. Organisms must be inoculated into deeper tissues, an anaerobic atmosphere is necessary for proliferation

Question 9

Actinomycosis (the oral cavity)

1. Describe this type of infection
2. What type of lesion is formed and name locations

Answer 9

1. Slow progressive, suppurative, fibrosing infection involving the jaw, thorax or abdomen
2. Formation of painful abscesses in the mouth, lungs or GI tract

Question 10

Actinomycosis (the oral cavity)

• Name*
• 2 uncommon conditions must occur to establish disease*

Answer 10

1. Organism must be inoculated into deeper tissues
2. An anaerobic atmosphere is necessary for proliferation

Question 11

Actiomycosis occurs at 4 distinct site

Answer 11

1. Cervicofacial – jaw injury, dental extraction or dental manipulation
2. Thoracic – caused by aspiration of the organisms contaminating dental debris
3. Abdominal – follows traumatic or surgical disruption of the bowel (especially the appendix)
4. Pelvic – associated with the prolonged use of IUD’s

Question 12

Actinomycosis (the oral cavity)

Macroscopical observation

Answer 12

The colonies appear as hard yellow grains* known as *sulfur granules

Question 13

Actinomycosis (the oral cavity)

Histological appearance:

Stain used:

Answer 13

The colonies appear as

rounded,

purple grains w/ scalloped eosinophilic borders* and *inflammatory infiltration

(H & E)

Question 14

Actinomycosis (the oral cavity)

Is considered what type of Inflammation?

Answer 14

Acute purulent inflammation

w/

abscesses formation

Question 15

Actinomycosis (the oral cavity)

Name risk factors

&

S/S, COMPLICATIONS

Answer 15

RF: Chronic tonsillitis, mastoiditis

S/S: painful abscesses in the mouth, pus, fever, swelling

complication: meningitis

Question 16

Actinomycosis (the oral cavity)

DESCRIBE: the appearance

Answer 16

1. Gram + hyphae, nutraphils, macrophages w/lipids and sometimes accompanied bacteria: rods & cocci
2. Foamyy macrophages (not like Makulicz cells-this name is reserved for Rhinoscleroma)
3. Amorphous dark purple area is the colony (actynomycetes) bacteria w/long branching filaments surrounded by neutrophils.