(P2) Dis. of Imm: Amyloidosis (the kidney) (H/E) (362); Congo Red (374) Flashcards
- Name this slide:
- Name the Stain:
- Define this condition:
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/690/q_image_thumb.jpg?1607269441)
1. Amyloidosis (the kidney)
2. H/E stain
3. It is a conditon associated with a number of inherited and inflammatory disorders in which exracellular deposits of fibrillar proteins are responsible for tissue damage and functional compromise.
Amyloid
Define its character:
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/693/q_image_thumb.jpg?1607269883)
is a complex of abnormal fibrils which are produced by the aggregation of misfolded proteins (which are soluble in their normal folded configurtion)
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/693/a_image_thumb.jpg?1607269996)
- TO SIMPLIFY:*
- group of diverse extravascular/extracellular protein deposits that have;*
- common pathomorphological properties*
- affinities for specific dyes*
- characteristic appearance under polarized light.*
- NAME THIS SLIDE
- This disease is primary or Secondary?
- It is associated with depositions of what material into where?
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/696/q_image_thumb.jpg?1607270148)
1. Amyloidosis (the kidney)
2. Secondary, non-inflammatory glomerulopathy
3. Associated with
Amyloid depositions within glomeruli
(sometimes other parts of the kidney)
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/696/a_image_thumb.jpg?1607270534)
Amyloidosis (the kidney)
- What 2 types of Amyloidosis*
- can be found in the kidneys?*
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/702/q_image_thumb.jpg?1607270844)
- AL- (Amyloid light chain) deposition of light chains of immunoglobulins
-
AA- (Amyloin-Associated) deposition of
* Alpha-amyloid* -amyloid fibril protein is derived from a unique non-Ig protein made by the liver
NAME THIS SLIDE
CLINICAL PRESENTATION:
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/703/q_image_thumb.jpg?1607270979)
Amyloidosis (the kidney)
- PROTEINURIA
- RENAL INSUFFICIENCY
- NEPHROTIC SYNDROME
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/703/a_image_thumb.jpg?1607271090)
NAME THIS SLIDE :)
Microscopic finding:
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/708/q_image_thumb.jpg?1607271135)
Amyloidosis (the kidney)
- Amyloid depositions within glomeruli
(sometimes depsitions may be found in renal parenchyma and within wall of blood vessels)
- In advanced disease lumina of arterioles in glomerulus and urinary space of Bowmans Capsule are narrowed
NAME THIS SLIDE
Name all structures that apply
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/712/q_image_thumb.jpg?1607271677)
Amyloidosis (the kidney)
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/712/a_image_thumb.jpg?1607271639)
- Amyloidosis (the kidney)*
- What is the chemical nature of the red material seen on this slide?*
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/713/q_image_thumb.jpg?1607271751)
fibril proteins
+
glycogen
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/713/a_image_thumb.jpg?1607271837)
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/719/q_image_thumb.jpg?1607271873)
Most commonly affected organs:
kidneys
heart
liver
GI
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/723/q_image_thumb.jpg?1607272016)
The type of protein involved?
ATTR (transthyretin- TTR)
- ATTR amyloidosis is a form of systemic amyloidosis* caused by amyloid deposits made up of a protein called transthyretin (TTR).
- ATTR amyloidosis can be either hereditary or acquired (non-hereditary).*
TTR is always present in the bloo_d, where it _transports thyroid hormone and vitamin A (retinol), hence the name: ‘trans-thy-retin’.
All the TTR in the blood is produced by the liver. TTR in the brain and the eye is made separately by a structure called the choroid plexus, which is located within the brain and produces the cerebrospinal fluid that bathes the brain and spinal cord.
NAME THIS SLIDE
Location of proteins?
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/724/q_image_thumb.jpg?1607272688)
Amyloidosis (the kidney)
extracellular
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/724/a_image_thumb.jpg?1607272649)
NAME THIS SLIDE
- define structures:
- What is happening to the Glomerular architecture?
- What is happening to the nucleus
- deposits of amyloid can be recognized as
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/729/q_image_thumb.jpg?1607272804)
- Amyloidosis (the kidney)*
- Amyloid in glomerulus (homogenous mass) Glomerular enlargement, architecture and c_apillary obliteration_, hypocellularity*
- nucleus is being squeezed to the side
- deposits of amyloid (pink around area)
- Amyloid in glomerulus (homogenous mass) Glomerular enlargement, architecture and c_apillary obliteration_, hypocellularity*
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/729/a_image_thumb.jpg?1607272854)
NAME THIS SLIDE
and
STAIN
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/732/q_image_thumb.jpg?1607274714)
Amyloidosis (the kidney)
(Congo red)
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/732/a_image_thumb.jpg?1607274790)
NAME THE STAINING
AMYLOID STAINS WHAT COLOR
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/734/q_image_thumb.jpg?1607274849)
CONGO RED
AMYLOID stains “RED”
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/734/a_image_thumb.jpg?1607275007)
Name this slide and Stain
- define the red-structure within the glomerulus
- what diagnostic method should be applied to this slide to confirm the nature of this lesion?
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/737/q_image_thumb.jpg?1607275650)
Amyloidosis (the kidney) (Congo Red)
- Amyloid
- polarized light with the use of
Thioflavin T (flourescent dye) for amyloid-under polarized light
Review: Thioflavins are fluorescent dyes that are available as at least two compounds, namely Thioflavin T and Thioflavin S. Both are used for histology staining and biophysical studies of protein aggregation
![](https://s3.amazonaws.com/brainscape-prod/system/cm/405/726/737/a_image_thumb.jpg?1607275554)