Nucleic acid synthesis

Question 1

Where does DNA replication happen?

Answer 1

At specific point called origin of replication site

Question 2

DNA helicase works by..

Answer 2

Unwind DNA strands by breaking H bonds

Question 3

DNA POLYMERASE can ONLY add DNA nucleotides to..?

Answer 3

to pre-existing chain of

nucleotides

Question 4

What does RNA primase do?

Answer 4

adds RNA PRIMER (short chain of nucleotides) at origin of

replication

Question 5

DNA polymerase starts replication by…

Answer 5

aligning complimentary nucleotides to

antisense strands in 5’ to 3’ direction

Question 6

What connects separated strands of DNA?

Answer 6

Ligase

Question 7

Continuous replication?

Answer 7

leading strand continuous addition of bases

by DNA polymerase from 5’ to 3’ direction.

Question 8

Discontinuous replication?

Answer 8

lagging strand in opposite direction, 3’ to
5’ direction.
RNA primer is placed beforehand. DNA polymerase then adds
complimentary bases in short fragments (okazaki fragments), which are later
enzymatically replaced to appropriate DNA molecules + bonded by DNA LIGASE
by formation of PHOSPHODIESTER BOND