Mammilian cell culture Flashcards
1
Q
Culture media components
A
organic nutrients, glucose, aa, vitamins, fa, fetal bovine serum, buffer, antibiotics, ph indicator
2
Q
Culture conditions
A
37 degrees, 5-10% co2 atmosphere and 90% humidity, glass or plastic ware
3
Q
Cell culture passaging
A
If density of cells too high, cells are split (passaged) in a flow box system
4
Q
Primary culture
A
direct from organism, can be frozen once, survive limited number of passages
5
Q
Immortalized cell line
A
unlimited number of passages, immortalized primary or derived from tumour
6
Q
Fully transformed cell line
A
all characteristics of malignant tumour
7
Q
Use of cell cultures
A
study cell cycle regulation, cancer progression, monoclonal antibody production, prenatal postnatal examination, human genome mapping, cloning etc.
