Detection Of Proteins

Question 1

SDS PAGE

Answer 1

Denatures proteins by binding to protein chain with its hydrocarbon tail and coats the chain with surfactant molecules

Question 2

Gel electrophoresis

Answer 2

Electricity to separate molecules

Larger molecules travel least distance

Question 3

Native gel electrophoresis

Answer 3

Detect abnormalities in Hb

Mutations-change in electric charge-diff mobility

Question 4

Immuno-staining

Answer 4

Antibody based method

Detect specific protein in sample

Question 5

Immunohistochemistry

Answer 5

Localising proteins in cell exploiting principle of antibodies binding to antigens in tissues.

Question 6

Immunity to chemistry

Answer 6

Intact cells have their extra cellular matrix removed.

Used to visualise localisation of antigen by using primary antibody which binds to it.

Question 7

Flow cytometry

Answer 7

Laser based cell sorting and counting by suspending cells in fluid and passing them by electronic detection apparatus

Question 8

ELISA

Answer 8

Enzyme linked immunosorbent assay

Measure conc of analyte

Question 9

Western blotting

Answer 9

Gel electrophoresis to separate native proteins by 3D structure or denatured protein by length of polypeptide. Proteins then transferred to membrane and stained with antibodies specific to target protein.