Mononegavirales: Rhabdoviridae

Question 1

What does Rhabdoviridae means?

Answer 1

(Rhabdos – rod, stick → plant rhabdoviruses)

Question 2

Morphology

Answer 2

-ssRNA genome

5nm Ø helical capsid
enveloped, ~70 × 180 nm
complete virions: bullet shaped (B);
incomplete: truncated (T)

Question 3

Resistance

Answer 3

Weak

Question 4

Biological properties

Answer 4

Baltimore V. – viral transcriptase

cell-bound infections

CPE: weak – IF is necessary
- Rabies: Negri bodies: inclusion bodies in the pyramidal cells

fish vesiculovirus: thermosensitive, 23°C optimum

zoonoses

Question 5

Antigenetic properties

Answer 5

cross reaction within genera

Question 6

Genus

Answer 6

Vesiculovirus genus
Lyssavirus genus
Ephemerovirus genus
Novirhabdovirus genus

Question 7

Diagnosis

Answer 7

IF, isolation + IF

experimental infection + IF

Question 8

Lyssavirus (Rabies)

Answer 8

(Lyssa - rabies)

Rabies
- genotypes: 
CVS (serotype-1), 
Lagos bat (serotype-2)
Mokola (serotype- 3)
Duvenhage (serotype-4) 
European bat 1, 2, (serotype 5 and 6)

• neuroinvasive – lethal encephalitis: behavior changes

urban rabies: dogs → humans
sylvatic rabies: fox, wolf, raccoon, bat
accidental hosts: cat, horse, sheep, cattle

Question 9

Ephemerovirus genus

Answer 9

Bovine ephemeral fever

• short fever – milk production ↓
• mosquito-transmitted (arbo) (Africa, Asia, Australia)

Question 10

Novirhabdovirus genus

Answer 10

Viral haemorrhagic septicaemia
- trout, Europe
Infectious haemopoetic necrosis
- salmon, Europe

Question 11

Vesiculovirus genus

Answer 11

Vesicular stomatitis virus

• ungulates, human – America
• clinical signs are similar to FMD