Mononegavirales: Filoviridae

Question 1

What does Filoviridae mean?

Answer 1

(filum – thread)

Question 2

Morphology

Answer 2

-ssRNA genome

80 × 800 – 10.000 nm helical capsid

enveloped

Question 3

Resistance

Answer 3

weak

Question 4

Biological properties

Answer 4

Baltimore V - viral transcriptase

Multiplicates in the cytoplasm ( similar to paramyxovirus and rhabdovirus)

CPE: cytoplasmic inclusion bodies

zoonoses! – bats are reservoirs

Question 5

Antigenetic properties

Answer 5

good antigens ↔ but rapid multiplication makes it a lethal disease
no cross-reactions

Question 6

Diagnosis

Answer 6

clinical symptoms
isolation
EM

Direct. RT - PCR
indirect: IF, ELISA

Question 7

Genus

Answer 7

Ebolavirus genus
Marburgvirus genus
Cuevavirus genus

Question 8

Ebolavirus genus

Answer 8

(Ebola - African river)

Ebola virus (Bundibugyo, Sudan, Tai Forest ebolavirus)

• human
• severe hemorrhage, encephalitis
• high mortality (> 90%)
• multiplicates in the cytoplasm, especially in organs as: Liver, spleen, lymph nodes and lungs.

Reston virus

• USA (Philippines)
• orphan?

Question 9

Marburgvirus genus

Answer 9

(Marburg – Germany)

Marburg virus

• monkey → human:
• fever
• meningoencephalitis

Question 10

Cuevavirus genus

Answer 10

(Cueva – cave)

Lloviu virus
- bat, Spain