Mononegavirales: Bunyaviridae

Question 1

Morphology

Answer 1

• segmented (3), -ssRNA genome
• helical capsid
• enveloped, ~ 100 nm

Question 2

Resistance

Answer 2

weak

Question 3

Biological properties

Answer 3

Baltimore V – viral transcriptase

arboviruses (except Hantavirus) – mosquito or tick transmission

zoonoses!

Question 4

Antigenic properties

Answer 4

• relatively good antigens

* cross-reaction within genera

Question 5

Diagnosis

Answer 5

• isolation, IF, EM
• indirect: VN
PCR

Question 6

Subfamilies

Answer 6

Peribunyaviridae
Phenuiviridae
Nairoviridae
Hantaviridae

Question 7

Peribunyaviridae

Answer 7

Orthobunyavirus genus

Question 8

Phenuiviridae

Answer 8

Phlebovirus genus

Question 9

Nairoviridae

Answer 9

Orthonairovirus genus

Question 10

Hantaviridae

Answer 10

Orthohantavirus genus

Question 11

Orthobunyavirus genus (Peribunyaviridae)

Answer 11

A lot of viruses! On the powerpoint following are marked red:

Akabane disease
•ruminants: congenital arthrogryposis, hydrencephaly
•mosquito-transmitted, Far-East, Australia, Israel

Inkoo virus
Tahyna virus
La Crosse virus
•human fever, rash, encephalitis (They all give the same symptoms)

Question 12

Phlebovirus genus (Phenuiviridae)

Answer 12

(Phlebotomidae - Sandfly)

Rift Valley fever
•sheep, cattle: abortion, fever, bloody diarrhea
•mosquito-transmitted, Africa

Question 13

Orthonairovirus genus (Nairoviridae)

Answer 13

(Nairobi)

Nairobi sheep fever
•sheep, goat: fever, gastroenteritis, abortion
•tick-transmitted (Africa, Asia) 
•Crimean–Congo haemorrhagic fever
•humans: hemorrhage, rash, nephritis

Question 14

Orthohantavirus genus (Hantaviridae)

Answer 14

(Hantaan – Korean region)

Hantaan hemorrhagic fever with renal syndrome
•human: fever, nephritis, high lethality
•reservoir: rodents (rat, deer mouse, cotton mouse, vole…)