Molecular and Biology Techniques Flashcards
ELISA Testing
Uses antigen-antibody interactions to determine if desired substance is present. Also enzyme linked included
Radioimmunoassay
Use radio labeled antibodies rather than enzyme linked antibodies
What result from ELISA testing tells if protein is present
A color change
Results of Radioimmunoassay
Radioactivity is measured and competition for antibodies takes place between antigen. Standard curve is formed based on radioactivity
Electrophoresis
Separates proteins by size. Electric current applied and samples migrate to positive pole according to size
Which molecules travel faster in electrophoresis
Smaller negative molecules
Blotting
transfer of DNA or proteins from an electrophoresis gel to a nitrocellulose of membrane to detect nucleic acid or protein
Southern Blotting
allows one to detect the presence of specific sequences within a heterogeneous sample of DNA. Isolate and purify target sequences of DNA
Northern Blotting
RNA is separated instead of DNA
What does Northern Blotting allow one to determine
if specific gene products are being expressed
What is Western Blotting used for
detect presence of certain proteins within a sample
How does western blotting differ from others
antibodies are used instead of nucleic acids
Eastern blotting
used to analyze post translational modifications of peptides
