Lecture 1 - Intro to Microbio Flashcards
Bacteriology
the study of bacteria
Mycology
the study of fungi
Parasitology
the study of parasitic worms & protozoa
Immunology
the study of immunity
binomial nomenclature
two-name naming system
(Genus species –> G. species
i.e. Escherichia coli –> E. coli)
3 Domains of Life
ABE
Archaea
Bacteria
Eukarya
4 Kingoms of Eukarya
Plants
Animals
Fungi
Protists
From Largest to Smallest, List the Categories in the Taxonomy Heirarchy
Domain
Kingdom
Phylum
Class
Order
Family
Genus
Species
Specific Epithet AKA
species
Prokaryotes include which two Domains?
Bacteria & Archaea
Staphylo-
clustered
(like grapes)
-coccus
spherical shaped
aureus
golden colored
(Au = gold)
Shape of bacillus
rod-like
Which is the genus and which is the species in the following name?
Staphylococcus aureus
Genus: Staphylococcus
Species: aureus
Eukaryotes
organisms whose cells have a nucleus & organelles
Prokaryotes
single-celled organisms; do not have a nucleus & organelles
Are viruses eukaryotes or prokaryotes? Why?
Neither. Because they are acellular (not made of cells).
acellular
not made of cells
Which is smaller? Bacteria or Viruses
viruses
Name Some Features of Viruses
- Acellular
- Nucleic Acid (can be DNA or RNA)
- Protein Coat
- External Membrane #envelope (only some viruses)
Inert
somewhere between living and non-living
What is the only way a virus can multiply? Why?
Viruses can only multiply by infecting other cells. Because they do not have the machinery to replicate on their own, they must use the machinery of a host cell.
virion
Any single, fully-formed virus that can infect a cell.
pathogen
disease causing entity
Which of the 3 Domains of Life is not known to cause disease in humans?
Archaea
Do prokaryotes have a nucleus?
No. They have a nucleoid region instead.
microbial ecology
studies the interactions of microbes with their environment
photosynthsis reaction
energy + 6 CO2 + 6 H2O –> C6H12O6 + 6 O2
energy + carbon dioxide + water –> glucose + oxygen
cellular respiration reaction
C6H12O6 + 6 O2 –> 6 CO2 + 6 H2O + energy
glucose + oxygen –> carbon dioxide + water + energy
Are algae eurkaryotic or prokaryotic?
eukaryotic
(have a nucleus and organelles)
Which Eukaryotic Kingdom do slime molds belong to?
Protists
Name one type of bacteria that can make their own food from their environment.
cyanobacteria
(blue pigment allows them to capture light and perform photosynthesis)
oxygenic
oxygen producing
C H O N P S
Carbon
Hydrogen
Oxygen
Nitrogen
Phosphorus
Sulfur
C
carbon
H
Hydrogen
O
Oxygen
N
Nitrogen
P
Phosphorus
S
Sulfur
6 Most Abundant Elements in Living Oranisms
C H O N P S
Bioremedation
using microbes to clean up pollutants/toxic wastes
(the microbe can eat/metabolize/breakdown the toxic substance into something that isn’t so harmful)
GMO
Genetically Modified Organisms
Biotechnology
using microorganisms to make useful products
Recombinant DNA
DNA from two species joined together
2 imporant Protein Drugs that are readily available now because of Recombinant DNA Technology
- Insulin
- Growth Hormone
plasmid
extrachromosomal DNA found in bacteria
Explain How Recombinant DNA Technology Works
- Plasmids are cut open using Restriction Enzymes #scissors
- DNA from another organisim is inserted in the opening
- Ligase Enzymes “glue” the two pieces of DNA together to form a circular piece of Recombinant DNA
- Recombinant DNA is inserted into the desired organism
Restriction Digest
scissors
Cutting DNA using Restriction Enzymes
Ligase
glue
Enzyme that joins pieces of DNA together
Fission
bacterial cell division/reproduction
Flora
normal microbiota
normal distribution of bacteria on the body and in the GI tract
2 Major Benefits of Our Normal Flora
- Immunity (low pH & competetive inhibition)
- B & K Vitamin Production
biofilm
micobial communities
(can be dry or wet) that are HIGHLY resistant to antimicrobial drugs & host immune system
quorum sensing
refers to how bacteria communicate with eachother and coordinate behavior within the matrix of the biofilm
Edward Jenner
milkmaid #smallpox #cowpox #vaccination
Discovered & Administered Vaccinations
Is smallpox considered eradicated?
Yes.
Louis Pasteur
5 Major Accomplishments
- Pasteurization
- Disproved Spontaneous Generation
- Aseptic Techniques (to only grow one type of bacteria in a culture)
- Microorganisms Cause Disease
- Showed some virulent bacteria can become avirulent over time #Chickens&Cholera
virulent
disease causing
avirulent
not disease causing
Explain Pasteur’s Chicken Cholera Experiment
Fresh cholera will kill a chicken. Aged culture of cholera will not kill a chicken and instead help it build immunity against fresh cholera.
Attenuated Microbes
Microbes that have lost their ability to confer disease #avirulent
Rudolf Virchow
Showed all cells come from other cells.
(omnis cellula e cellua)
Hansen’s Disease AKA
lepracy
Phenol AKA
Carbolic Acid
Used phenol (carbolic acid) to sterilize wounds
Jospeh Lister
Koch’s Postulates
BacillusAnthracis–>anthrax
specific microbes cause specific diseases
Robert Koch
Developed Koch’s Postulates stating that specific microbes cause specific diseases
Alexander Flemming
discovered penicillin
Paul Ehrlich
Salvarsan for Syphillis
#MagicBullet
Salvarsan is used to treat ______.
Syphillis
Infectious Diseases
a pathogen invades a susceptible host
Plasmodium causes _______.
Malaria
Prion
protiens that can cause disease
Noninfectious Disease
diseases that are not caused by pathogens and cannot be passed from one person to another
Emerging Infectious Diseases
diseases that are breaking out in larger numbers than usual (can be new or old diseases)
Which Eukaryotic Kingdom do algae belong to?
Protists
Which Eukaryotic Kingdom are molds and yeasts part of?
Fungi
Which Eukaryotic Kingdom are mosses a part of?
Plants
Which Eukaryotic Kingdom are worms a part of?
Animals
What is the difference between the cell walls of bacteria and archaea?
Bacterial cell walls contain PEPTIDOGLYCAN.
Arachaea cell walls DO NOT contain peptidoglycan.
helminths AKA
worms
What type of microscope must be used to see viruses?
Electron Microscope
What is one example of a Biogeochemical Cycle?
Nitrogen Cycle
Two Genus of Bacteria Particularly Useful in Bioremediation
Bacillus
Pseudomonas
What is Saccharomyces cerevisiae?
a species of yeast which feeds on sugar and is used to make beer
Plants are photosynthetic and can use the energy from the sun to produce sugar and O2. Can bacteria also be photosynthetic?
Yes.
i.e. Cyanobacteria
cyan
blue
Bacteria that Produces Bt Toxin
Bacillus thuringiensis
Prior to the advent of scientists creating genetically modified organisms, where did Human Growth Hormone (HGH) Rx come from?
cadavers
Prior to the advent of scientists creating genetically modified organisms, where did insulin Rx come from?
Slaughtered Pigs
What is the ratio of bacteria on our bodies to our total cells?
We are outnumbered 10:1
Which bacteria is often the culprit for causing dental caries (cavities)?
Streptococcus mutants
Causes Anthrax
Bacillus anthracis
Chemotherapy
Treatment of disease with chemicals taken into the body
Malaria is caused by
Plasmodium
Aseptic Techniques
procedures used to prevent microbial contamination & infection