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Histology > Lecture 1 - cell types and slide prep > Flashcards

Lecture 1 - cell types and slide prep Flashcards

1
Q

TEM

A

Transmission electron microscope. focus a beam of electrons. X500,000

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2
Q

SEM

A

Scanning electron microscope. X100,000. Can see the topology

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3
Q

H&E stain (Hematoxylin and Eosin)

A

Nucleus and cytoplasm, respectively

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4
Q

PAS

A

glycogen, glycoproteins

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5
Q

Aldehyde Fuchsin

A

elastic fibers, CT

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6
Q

Orcein

A

collagen fibers, cytoplasmic counterstain, ELASTIC FIBERS

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7
Q

Silver

A

Golgi apparatus, reticular fibers

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8
Q

Freeze Fracturing

A

able to split the lipid bilayer into two.

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9
Q

P Face and E Face

A

P face - protoplasmic

E face - external face

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10
Q

Formalin (fixation)

A

cross links to proteins to keep a specimen in place during prepartion

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11
Q

Four basic types of tissues

A

Nervous, Epithelial, muscle, and connective

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12
Q

Basal lamina contents

A

Type IV collagen, glycoproteins laminin and entactin, and herapin sulfate.

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13
Q

Simple vs stratified

A

Simple - one layer of cells

Stratified - multiple layer of cells

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14
Q

Squamous

A

flat.

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15
Q

Cuboidal

A

Square.

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16
Q

Columnar

A

rectangular

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17
Q

Simple squamous examples

A

Examples are mesothelium lining the pleural, pericardial and peritoneal cavities, and the endothelium lining the cardivoascular system

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18
Q

Simple cuboidal examples

A

Examples are conducting passageways (glandular ducts), or play a role in secretion or absorption (Distal convoluted tubules and collecting ducts in kidneys)

19
Q

Simple columnar examples

A

small intestine (gall bladder) and respiratory tract

20
Q

Transitional epithelium

A

bladder, extends when full of urine but collapses when urine is absent.

21
Q

Pseudostratified epithelium and an examples

A

every cell makes basement membrane contact (respiratory or male uterine tract). Found in the trachea and epididymis

22
Q

Stratified squamous example

A

skin.

23
Q

Keratinized

A

Dead skin cells found on top. Found in external surfaces of the body

24
Q

Stratified cuboidal example

A

sweat gland duct

25
Q

Stratified columnar example

A

salivary gland duct and the male urethra

26
Q

Where are binucleated cells located

A

bladder cells which is transitional epithelium

27
Q

Parenchyma

A

secretory cells of the gland

28
Q

Stroma

A

CT within the gland, which supports the secretory cells

29
Q

Exocrine glands

A

possess ducts that go to the surface of the body or into the cavity of a hollow organ

30
Q

Endocrine glands

A

secret into blood vessels

31
Q

simple vs compound gland

A

simple: one unbranched duct
Compound: ducts branch

32
Q

Acinar vs tubular secretory portion

A

Acinar is a bulb shape.

Tubular is elongated

33
Q

Mucous secretion

A

muco-poly saccharides which do not stain well thus produce a lighter stain

34
Q

Serous secretion

A

Contain more proteins and will stain darker. Also usually contain more water

35
Q

Merocrine secretion

A

secreting vesicles bud off of golgi apparatus and then fuse with the apical membrane and relase into extracelluluar environment

36
Q

Apocrine secretion

A

A large chunk of the cell is secreted. Seen in mammary glands

37
Q

Holocrine secretion

A

A whole cell is secreted. Such as the sebaceous gland of the skin.

38
Q

Brush border is made up of what and found where

A

microvilli and found in the small intestine

39
Q

Microvilli proteins

A

actin filament both into the body of the microvilli but also perpendicular in the basal area to provide rigidity. Fimbrin and villin also found

40
Q

Cilia

A

Found in the respiratory system (trachae). Thick and longer than cilia. Can see an axoneme

41
Q

Stereocilia

A

Skinny microvilli and contain actin. Seen in ductus epididymis.

42
Q

Microplicae

A

surface folds. Help retain mucus and other solutions. Found in GI tract

43
Q

Goblet cell and type of secretion

A

secreting cells of mucous into the lumen of the intestine. Merocrine type of secretion.

Histology (20 decks)

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