LAT Review

Question 1

Which entity in AAALAC International approves an institution’s application for accreditation?

Answer 1

council on accreditation

Question 2

How many US Government Principles for the Utilization and Care of Vertebrate Animals Used in Testing, Research, and Training are there?

Answer 2

9

Question 3

What person has the responsibility of ensuring the institution complies with the Animal Welfare Act?

Answer 3

chief executive officer

Question 4

A cost recovery analysis for technical services offered by the vivarium is based on what?

Answer 4

Revenue for the service compared to expenses for related supplies and labor

Question 5

What can the multiplex fluorescent immunoassay detect?

Answer 5

Presence of viral antibodies

Question 6

What type of chemical responds strongly in the presence of water?

Answer 6

reactive

Question 7

Serological tests conducted on samples taken when animals arrive at the facility provide information about their health status at which period of time?

Answer 7

2 weeks before shipment

Question 8

The cost for personnel generally represents what percentage of an animal facility’s budget?

Answer 8

more than 60%

Question 9

What is a common method used to detect exposure to viral pathogens?

Answer 9

ELISA

Question 10

How many days does it generally take for an animal to develop an immune response to an agent?

Answer 10

10-14

Question 11

Which ABSL level relates to infectious agents that can cause serious or potentially lethal diseases when inhaled?

Answer 11

ABSL-3 and higher

Question 12

What USDA publication clarifies the intent of the Animal Welfare Act?

Answer 12

Animal Care Policy Manual

Question 13

PCR rodent infectious agent (PRIA) testing allows swift identification of environmental pathogens by detecting what?

Answer 13

Pathogen genetic material

Question 13

In which phase of the ADDIE instructional design model does the needs assessment take place?

Answer 13

Analyze

Question 14

What management function most directly affects accomplishment of the mission of the institution and animal care program?

Answer 14

goal setting

Question 15

Which agency administers the Animal Welfare Act?

Answer 15

Animal Plant and Health Inspection Service

Question 16

How often should a research facility using regulated species update their USDA registration?

Answer 16

every 3 years

Question 17

Which institutional office generally has the responsibility for monitoring the handling of radioactive materials in the laboratory?

Answer 17

Radiation Safety Officer

Question 18

Along with the Nuclear Regulatory Commission, who administers the licensees of nuclear material?

Answer 18

Agreement States

Question 19

A postmortem cecal exam is used to detect what species?

Answer 19

Aspiculuris

Question 20

If bleach is mixed with a cleanser containing _______ , irritating, volatile compounds are produced.

Answer 20

ammonia or acids

Question 21

What is an appropriate reason for using static tanks for fish?

Answer 21

Isolating fish for a short term

Question 22

The temperature of a diluted liquid sanitizer should not be increased due to:

Answer 22

pH stability

Question 23

The rate of air flow from a vent is measured using which device?

Answer 23

Anemometer

Question 24

What metal, often found in municipal water, can be toxic to aquatic animals?

Answer 24

copper

Question 25

What is the purpose of a pass box?

Answer 25

To promote the direction of airflow from the animal room to the hallway

Question 26

Xenopus is the genus of which species?

Answer 26

African clawed frogs

Question 27

What is the typical exposure time for formaldehyde decontamination?

Answer 27

12 hours

Question 28

What is the term for a passageway with two locking doors that cannot be opened simultaneously?

Answer 28

airlock

Question 29

What is the purpose of a pass box?

Answer 29

To transfer containers of supplies into and out of the facility

Question 30

Which document recommends that all social species be housed in pairs or groups in a laboratory setting?

Answer 30

The Guide

Question 31

Germicides may be used on live animals and are therefore regulated by what agency?

Answer 31

The FDA

Question 32

Which biosafety level contains indigenous organisms that pose a moderate risk of disease?

Answer 32

2

Question 33

What is the lowest biosafety level at which personnel must receive training on how to safely work with the hazardous agent?

Answer 33

BSL-2

Question 34

What factor lowers the pH in an aquatic system?

Answer 34

carbon dioxide

Question 35

What movement is described by bending or folding (decreasing the angle)?

Answer 35

flexion

Question 36

What animals have an additional lymphoid organ not present in mammals called the bursa of Fabricius?

Answer 36

birds

Question 37

A 5% alcohol solution by volume would be a solution made from 5 mL of alcohol and enough solvent to bring the total volume up to ____ mL.

Answer 37

100

Question 38

Where does the breakdown of cellulose occur in rabbits, horses, and rodents?

Answer 38

the cecum

Question 39

What is the process by which DNA is inserted into a cell in homologous recombination?

Answer 39

Electroporation

Question 40

What animal has thrombocytes that lack a nucleus?

Answer 40

rat

Question 41

The hormones epinephrine and norepinephrine are produced by the:

Answer 41

adrenal medula

Question 42

Antibodies are produced by what cells?

Answer 42

B Cells

Question 43

What term describes a situation where new genes are inserted by a targeted mutation?

Answer 43

knockin

Question 44

In the rederivation process a female that has been tested and found free of pathogens is known as:

Answer 44

clean

Question 45

What are the multi-branched extensions of a neuron?

Answer 45

dendrites

Question 46

What is the structure in which neurons extending from the brain and neurons extending to the tissue are connected?

Answer 46

ganglia

Question 47

What type of test is done to verify the health status of recipient females for embryo rederivation?

Answer 47

serology

Question 48

What step must be taken to produce a chimera with hopes of achieving germline transmission?

Answer 48

Implantation of injected blastocysts

Question 49

True or False: Blood is a connective tissue.

Answer 49

True

Question 50

What provides strength to the connective tissue found in tendons and ligaments?

Answer 50

collagen

Question 51

What is the area where growth occurs in the long bone in a young animal?

Answer 51

Epiphysis

Question 52

In what structure is an egg transported from an ovary to the uterus?

Answer 52

Salpinx

Question 53

What step must be taken to produce a chimera with hopes of achieving germline transmission?

Answer 53

Implantation of injected blastocysts

Question 54

What is the substrain of C57BL/6J?

Answer 54

6

Question 55

Subcutaneous injections are made in what structure?

Answer 55

hypodermis

Question 56

In mammals, thrombocytes are:

Answer 56

platelets

Question 57

What structure is an egg transported from an ovary to the uterus?

Answer 57

Salpinx

Question 58

What supplies O2 and other nutrients to the heart itself?

Answer 58

coronary arteries

Question 59

Where does the breakdown of cellulose occur in rabbits, horses, and rodents?

Answer 59

in the cecum

Question 60

The ankle joint is known as the _______ in quadrupeds.

Answer 60

hock

Question 61

Muscles are attached to bones by:

Answer 61

tendons

Question 62

How do adult Xenopus respire?

Answer 62

breathing with lungs

Question 63

What does the layer of muscle cells do for an artery?

Answer 63

changes the diameter

Question 64

What colony is bred to establish a colony with the desired DNA?

Answer 64

the foundation colony

Question 65

What test is done to verify the health status of recipient females for embryo rederivation?

Answer 65

serology

Question 66

What type of solutions are used to represent the concentration of electrolytes in sterile fluids used for injection?

Answer 66

normal

Question 67

What type of cells break down pathogens that have been trapped in the lymph nodes?

Answer 67

macrophages

Question 68

What type of disease is defined as one with a sudden outbreak that affects a large number of animals?

Answer 68

Epizootic

Question 69

What condition in frogs results from an infection or a metabolic disorder and can impair their equilibrium in water?

Answer 69

bloat

Question 70

A newborn’s temporary immunity is known as _______ immunity.

Answer 70

passive

Question 71

When an animal has an allergy, its immune system recognizes the allergen as a(n):

Answer 71

antigen

Question 72

Infection with what human disease is potentially fatal to marmosets?

Answer 72

herpes simplex

Question 73

What is an animal’s primary means of keeping out most invading organisms?

Answer 73

skin and mucus membranes

Question 74

What practice is most commonly adopted to address rodents infected by a disease agent?

Answer 74

euthanasia

Question 75

What type of testing has been adopted by many facilities in place of sentinel testing?

Answer 75

Molecular techniques

Question 76

What do white chalky deposits on the sides of the tank indicate?

Answer 76

high hardness level

Question 77

How often should water hardness be monitored?

Answer 77

weekly

Question 78

What species is often used for determining the chemical safety of pesticides and industrial chemicals?

Answer 78

fish

Question 79

Disruption of power supply may significantly deplete the ________ in the system water.

Answer 79

dissolved oxygen

Question 80

What type of filter removes large, solid waste?

Answer 80

mechanical

Question 81

What mandate states that protocols for USDA-covered species may only be approved for 1 year?

Answer 81

Animal Welfare Regulations

Question 82

What are the clusters of cells that secrete insulin and glucagon?

Answer 82

The islets of Langerhans

Question 83

What movement is described by bending or folding (decreasing the angle)?

Answer 83

flexion

Question 84

To collect sperm from a male zebrafish, what is avoided by ensuring that the urogenital pore is dry?

Answer 84

activation of sperm

Question 85

What macaque species are the most commonly investigated Old World monkeys in behavioral research?

Answer 85

M. mulatta

Question 86

What is an appropriate reason for using static tanks for fish?

Answer 86

isolating for a short term

Question 87

What is the benefit of using the dry process of VHP instead of the wet process?

Answer 87

It reduces the corrosiveness of the compound.

Question 88

Who can restrict or withdraw approval of the Animal Welfare Assurance if an institution fails at its responsibilities?

Answer 88

OLAW

Question 89

An animal’s total blood volume is approximately what percentage of its body weight?

Answer 89

6%

Question 90

How are scissors classified?

Answer 90

by blade tips

Question 91

What comprises the endocrine system?

Answer 91

glands

Question 92

What is produced by the action of luciferin on the luciferase gene?

Answer 92

light

Question 93

What do polyclonal antibodies react against?

Answer 93

Multiple unique areas on a particular antigen

Question 94

Which state passed legislation starting in 2020 that prohibits the sale of cosmetic products and ingredients that have been tested on animals?

Answer 94

California

Question 95

What is an animal called that has some cells containing the new gene and some cells without the new gene?

Answer 95

chimera

Question 96

What type of test involves repeated administration of a substance over 28 days (1 month)?

Answer 96

subacute

Question 97

How effective are HEPA filters in removing particulate matter from air?

Answer 97

99.97%

Question 98

It is recommended that adult mice receive retroorbital injections no greater than what volume?

Answer 98

150 microliters

Question 99

What is the matrix in a mature bone?

Answer 99

Nonliving material containing minerals

Question 100

What process allows researchers to know whether an animal possesses a specific gene?

Answer 100

Electrophoresis

Question 101

In many animals what percent of the blood is comprised of plasma?

Answer 101

55%

Question 102

What is the maximum number of surgeries for oocyte collection recommended over the life of a Xenopus?

Answer 102

6

Question 103

What type of tissue lines the heart and blood vessels?

Answer 103

endothelium

Question 104

How does the Whitten Effect assist in timed mating?

Answer 104

It can produce a group of females for mating at one time.

Question 105

The lack of both cellular and antibody immune response makes SCID mice good models for __________ .

Answer 105

tumors

Question 106

In what species is the Draize test most commonly used?

Answer 106

rabbits

Question 107

What may be given to amphibians to stimulate egg production?

Answer 107

Gonadotropins

Question 108

What enzyme is used in bioluminescence imaging?

Answer 108

Luciferase

Question 109

After how many consecutive litters that failed to thrive should rodent breeders be retired?

Answer 109

2

Question 110

Both too much and too little of specific vitamins and minerals can result in what type of disease?

Answer 110

nutritional

Question 111

In Segment III studies, how many pairs of pups are selected from each litter to produce an F1 generation?

Answer 111

1

Question 112

What mice lack both T cells and B cells?

Answer 112

SCID mice

Question 113

What metal, often found in municipal water, can be toxic to aquatic animals?

Answer 113

copper

Question 114

What factor lowers the pH in an aquatic system?

Answer 114

carbon dioxide