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Biol3 > LabEx2: 9,10,11,12,13 > Flashcards

LabEx2: 9,10,11,12,13 Flashcards

1
Q

Lysozyme is an ezyme found in body’s secretion, such as ___1___,____2____,____3_____ and ____4___.

A
  1. saliva, 2. nasal secretion, 3. tears and
  2. hen’s egg(only the concentratedwhite part)
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2
Q

Lysozyme hydrolyzes the glycosidic bond between the ____1____ units of bacterial cell wall. which can cause the bacterial cell to ____2____.

A
  1. NAG-NAM (backbone components)
  2. Osmotic bursting
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3
Q

Lysozyme action is more pronounced and clear in Gram ___1____ than Gram ___2_____

A
  1. Gram Positive
  2. Gram Negative
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4
Q

Gram _______ cells susceptibility to lysozyme action is due to lack of a Protective barrier to the peptidoglycan layer.

A

Gram positive

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5
Q

In Gram ______ cells have an outer membrane which covers the peptidoglycan layer and shields from enzymatic attack of lysozyme

A

Gram Negative

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6
Q

__________ and __________, they represent media which routinely used by microbiologist to isolate, identify and confirm presence of specific microorganism from specimen or environment

A

Starch hydrolysis test:

Selective and differential media

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7
Q

______ have components that inhibit the growth of unwanted bacteria and encourage growth of desired microorganism. Example: Thayer Martin media,Sabouraud’s dextose Agar

A

Starch hydrolysis test:

Selective media

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8
Q

________ designed for easier detection and isolation of microorganism of interest from other growing on the same plate. Often a particular trait of the microbes is exploited

A

Starch hydrolysis test:

Differential media

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9
Q

EMB Agar, Mannitol salt Agar and Mc Conkey Agar are both ________ and _______ media

A

Starch hydrolysis test:

Selective and differential

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10
Q

_______ is a complex carbohydrates (polysaccharide), observed as inclusion bodies in some bacteria.

A

Starch hydrolysis test:

Starch

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11
Q

______ serves as carbon and energy reserve and can be degraded into individual glucose units and metabolized

A

Starch hydrolysis test:

Starch

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12
Q

If Bacteria are cultured on media containing starch as sole sugar source, some synthesize and secretes an enzyme called _______

A

Starch hydrolysis test:

Amylase

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13
Q

_______ belongs to the class of exoenzyme and breaks down starch so individual glucose unit can be transported inside cell easily.

A

Starch hydrolysis test:

Amylase

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14
Q

The ability to break down starch is examined using ________, it detects presence or absence of intact starch around Bacterial growth area

A

Starch hydrolysis test:

Iodine reagent

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15
Q

If starch is broken vicinity of bacterial growth, there would be…..

A

Starch hydrolysis test:

No reaction of iodine with starch

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16
Q

In starch hydrolysis test, Indication of starch hydrolysis by amylase, is seen as a __________ around bacterial growth

A

Starch hydrolysis test:

Clear area (halo)

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17
Q

In starchhydrolysis test, If intact starch is observed as ___________ around colony which indicate as non-hydrolysis

A

Starch hydrolysis test:

Blue Color/Dark coloration

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18
Q

_______ is an enzyme produced by many microbes to neutralize the toxic effect of hydrogen peroxide.

A

Catalase Test:

Catalase

19
Q

Catalase breaks down hydrogen peroxide to generate ______ and _________.

A

Catalase Test:

water and oxygen

20
Q

In catalase test, oxygen release from the peroxide molecule is abserved as ________. immediate ______ is indicative of positive catalase test

A

Catalase Test:

Bubble

bubbling

21
Q

Bacterial cells growth is influenced by many ______ and ________ factors

A

Anaerobic bacterial growth/Gas Chamber:

physical and chemical

22
Q

______ and chemical factors are required at specifc levels; any deviations from their normal levels and conditions compromises severely bacterial growth

A

Anaerobic bacterial growth/gas chamber:

Physical and chemical

23
Q

In anaerobic chamber, available oxygen is depleted due to chemical reactions which are initiated by ________.

A

Anaerobic bacterial growth/gas chamber:

adding water

24
Q

The bacteria Clostridium sporogenes is a aerobic or anaerobic?

A

Anaerobic bacterial growth/gas chamber:

Anaerobic

25
Q

the bacteria Bacillus subtiles a aerobic or anaerobic?

A

Anaerobic bacterial growth/gas chamber:

aerobic

26
Q

The bacteria staphylococcus epidermidis is aerobic or anaerobic?

A

Anaerobic bacterial growth/gas chamber:

aerobic

27
Q

________ is primarily used to differentiate enterics from nonenterics gram negative bacilli.

A

TSI agar (triple sugar iron)

28
Q

_____ a complex, enriched medium with 3 sugars (glucose, sucrose and lactose).

A

TSI agar (triple sugar iron)

29
Q

In TSI agar (triple sugar iron), _______ is supplemented at the low range concentration (0.1%) compared to the other 2 sugars.

A

Glucose

30
Q

TSI agar (triple sugar iron) Medium differentiates bacteria on the basis of ______ Fermentation and ______ Reduction.

A

Sugar fermentation and sulfur reduction

31
Q

TSI agar (triple sugar iron) media that has a deep butt is ________.

A

anaerobic condition

32
Q

TSI agar (triple sugar iron) media that has a shallow slant is ______. due to Na2S2O3–>H2S

A

aerobic condition

33
Q

In TSI agar (triple sugar iron) media yellow slant/butt, ______, _______, and/or _______ fermentation with acid accumulation in slat and butt.

This detects bacteria E.coi and Enterobacter aerogenes which also contain gas production due to cracks and lifting agar.

A

Glucose and lactose and/or sucrose

34
Q

In TSI agar (triple sugar iron) media Red slant/ Red Butt, No Fermentation. _______ catabolized aerobically and anerobically with alkaline products. if its a Red Butt, is most likely a Obligate aerobe.

This detects bacteria Pseudomonas aeroginosa

A

Peptone

35
Q

In TSI agar (triple sugar iron) media Black precipitate in the gar, _____ reduction. Hydrogen sulfide (H2S) maybe produced by the reduction of _________ in the medium.

This detects bacteria Proteus mirabilis, also have a red slant which due to glucose.

A

Sulfer Reduction

thiosulfate

36
Q

______ physical factor is used extensively for microbial growth control. most vegatative cells of microbes exhibit differences in their _____ tolerance. _______ can be in the form of moist _____ or dry ________.

A

Control of microorganisms:

Heart

37
Q

______ is used in, pasteurization process (milk and juices), autoclaving of surgical materials and sterilization of baby’s feeding bottles.

A

Control of microorganisms:

Moist heart

38
Q

________ can be used to kill microbes, sterilization of microbiological inoculating loop in lab.

A

Control of microorganisms:

Dry Heart

39
Q

________ form of cells get killed by moist heart.

A

Control of microorganisms:

Vegetative

40
Q

During control of microorganisms Moist Heart lab, the bacteria ___1____ did not grow, but ____2____ and _____3__ have grown from 5-25min.

A
  1. E.coli
  2. staphylococcus epidermidis
  3. bacills cereus
41
Q

____1_____ is a method that incorporates mechanical removal of microorganisms from samples. the method involves passage of a liquid through a screen like material of ___2_____.

A
  1. Filter sterilization
  2. high tensile strength
42
Q

In filter sterilization, the filter paper is inert; is made of _________ or ________.

A

Nitrocellulose or PVDF

43
Q

In filter sterilization, Heat sensitive preparations of antibiotic, glucose, vaccines, ophthalmic soulution and enzymes are made ___1____. the pore size of the membrane is variable; we will use membrane with pore size of ___2___.

A
  1. free of microbes (sterilized)
  2. 0.45um
44
Q

In filter sterilization, uses filter water sample to evaluate presence of coliforms, it yields accurates results if the colony count is in range of ______ organisms per filter disces. filtration can be used on emb plate or agar plate

A

30-300

Biol3 (26 decks)

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