Histology Flashcards
Define histology
Is the branch of anatomy that studies tissues of animals and plants involving scientific disciplines
List 6 basic histological terminology
cell biology
biochemistry
physiology
embryology
gross anatomy
pathology
List 3 histological sectioning planes
Oblique section
Longitudinal section
Cross (transverse) section
What are the 6 steps of paraffin wax sectioning
Fixation
Dehydrate
Embedding
Sectioning
Dried
De waxing
What is routine H&E staining
Haematoxylin and eosin stain is used for tissue specimens to reveal the underlying tissue structures and conditions
What type of dye is harmatoxylin
Basic dye
What type of dye is eosin
Acid dye
What colour does haematoxylin show up as
Purplish blue colour
What colour does eosin show up as
Reddish or pink
What is the direct method of immunocytochemistry
Uses a single antibody directed against the target of interest
The primary antibody is directly conjugated to a fluorophore
What is the indirect method of immunocytochemistry
Uses two antibodies
Primary antibody is unconjugated and a fluorophore conjugated secondary antibody directed against the primary antibody is used for detection
What type of staining is used for bacteria
Gram stain
What type of section is used for hard tissues
Ground section
What does PAS staining stand for
Periodic acid schiff staining
How do you know when the gram stain is positive
If the bacteria stays purple
