Histology Flashcards
Histopathology
The study of changes in microscopic anatomy caused by disease
What are the steps needed to prepare the slide
Fixation, Selection and trimming, processing and embedding (e.g in paraffin wax) then sectioning thin slices and staining
Haemotoxylin
(purple/blue) a basic dye, can bind to nucleic acids
Eoisin
Acidic dye, binds to bases e.g most proteins in the cytoplasm
What are the 4 different types of tissue in the body?
Epithelium, Connective tissue, Muscle and nervous tissue
What does the basement membrane act as?
a selective barrier, blood and lymph vessels cannot penetrate
What two forms does epithelium exist in?
Surface and glandular
What can surface epithelium split into?
Simple, stratified and pseudostratified
What are immunohistochemical stains?
They use antibodies to bind to a tissue-specific substance, and a second labelled antibody binds to the first
What are the labels that attach to antibodies
coloured dye or a fluorescent marker
What is connective tissue derived from?
from the mesoderm and it forms a matrix underneath the epithelium
What mostly makes up the extracellular matrix (connective tissue)
Protein fibres and ground substances
What is the function of connective tissue?
Provide structural support to other tissues, mediate the exchange of nutrients and waste
What are the types of fibres found in connective tissue?
Collagen and Elastic
What are Collagen fibres composed of?
Composed of collagen, have a great tensile strength
